Journal article
Myosin-Va restrains the trafficking of Na+/K+-ATPase-containing vesicles in alveolar epithelial cells
Journal of cell science, Vol.122(21), pp.3915-3922
10/06/2009
DOI: 10.1242/jcs.046953
PMCID: PMC2773192
PMID: 19808891
Abstract
Stimulation of Na
+
/K
+
-ATPase activity in alveolar
epithelial cells by cAMP involves its recruitment from intracellular
compartments to the plasma membrane. Here, we studied the role of the actin
molecular motor myosin-V in this process. We provide evidence that, in
alveolar epithelial cells, cAMP promotes Na
+
/K
+
-ATPase
recruitment to the plasma membrane by increasing the average speed of
Na
+
/K
+
-ATPase-containing vesicles moving to the cell
periphery. We found that three isoforms of myosin-V are expressed in alveolar
epithelial cells; however, only myosin-Va and Vc colocalized with the
Na
+
/K
+
-ATPase in intracellular membrane fractions.
Overexpression of dominant-negative myosin-Va or knockdown with specific shRNA
increased the average speed and distance traveled by the
Na
+
/K
+
-ATPase-containing vesicles, as well as the
Na
+
/K
+
-ATPase activity and protein abundance at the
plasma membrane to similar levels as those observed with cAMP stimulation.
These data show that myosin-Va has a role in restraining
Na
+
/K
+
-ATPase-containing vesicles within intracellular
pools and that this restrain is released after stimulation by cAMP allowing
the recruitment of the Na
+
/K
+
-ATPase to the plasma
membrane and thus increased activity.
Details
- Title: Subtitle
- Myosin-Va restrains the trafficking of Na+/K+-ATPase-containing vesicles in alveolar epithelial cells
- Creators
- Emilia Lecuona - Northwestern UniversityAlexander Minin - Northwestern UniversityHumberto E. Trejo - Northwestern UniversityJiwang Chen - Northwestern UniversityAlejandro P. Comellas - Northwestern UniversityHaiying Sun - Northwestern UniversityDoris Grillo - Northwestern UniversityOxana E. Nekrasova - Northwestern UniversityLynn C. Welch - Northwestern UniversityIgal Szleifer - Northwestern UniversityVladimir I. Gelfand - Northwestern UniversityJacob I. Sznajder - Northwestern University
- Resource Type
- Journal article
- Publication Details
- Journal of cell science, Vol.122(21), pp.3915-3922
- Publisher
- Company of Biologists
- DOI
- 10.1242/jcs.046953
- PMID
- 19808891
- PMCID
- PMC2773192
- ISSN
- 0021-9533
- eISSN
- 1477-9137
- Language
- English
- Date published
- 10/06/2009
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; ICTS; Internal Medicine
- Record Identifier
- 9984359930802771
Metrics
12 Record Views