N-acetyl-L-cysteine increases MnSOD activity and enhances the recruitment of quiescent human fibroblasts to the proliferation cycle during wound healing

Gaowei Mao; Monali Goswami; Amanda L Kalen; Prabhat C Goswami; Ehab H Sarsour

doi:10.1007/s11033-015-3935-1

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N-acetyl-L-cysteine increases MnSOD activity and enhances the recruitment of quiescent human fibroblasts to the proliferation cycle during wound healing

Journal article

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N-acetyl-L-cysteine increases MnSOD activity and enhances the recruitment of quiescent human fibroblasts to the proliferation cycle during wound healing

Gaowei Mao, Monali Goswami, Amanda L Kalen, Prabhat C Goswami and Ehab H Sarsour

Molecular biology reports, Vol.43(1), pp.31-39

01/2016

DOI: 10.1007/s11033-015-3935-1

PMCID: PMC4822548

PMID: 26671656

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https://www.ncbi.nlm.nih.gov/pmc/articles/4822548View

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Abstract

The rebuilding of the connective tissue during wound healing requires the recruitment of fibroblasts to the wound area as well as reentry of quiescent fibroblasts to the proliferative cycle. Whether this process can be modulated by a small molecular weight thiol antioxidant N-acetyl-L-cysteine (NAC) was tested in normal human skin fibroblasts (NHFs) using a uni-directional wound healing assay. NAC treated cells demonstrated a decreased migration rate but increased number of proliferating cells recruited into the wound area post wounding. Fifteen day quiescent control and NAC treated NHFs were re-plated at a lower density and cell numbers counted at different days post-plating. Interestingly, NAC treated cells exhibited increased cellular proliferation indicated by both decreased cell population doubling time and increased S phase cells. NAC treated cells demonstrated decreased steady state levels of reactive oxygen species as well as increased protein and activity levels of manganese superoxide dismutase (MnSOD). NAC treatment failed to induce proliferation in quiescent cells lacking MnSOD expression. These results demonstrate that NAC enhanced the recruitment of quiescent NHFs into proliferation cycle during wound healing. Our results also suggest that the wound healing properties of NAC might be due to its ability to induce and enhance MnSOD expression and activity. Altogether, these findings suggest NAC might be potentially developed as a dietary intervention to improve tissue injury in animals and humans.

Reactive Oxygen Species - metabolism

Oxidation-Reduction

Humans

Antioxidants - pharmacology

Mice, Knockout

Animals

Fibroblasts - drug effects

Acetylcysteine - pharmacology

Cell Proliferation - drug effects

Fibroblasts - cytology

Mice

Cell Cycle - drug effects

Wound Healing - drug effects

Superoxide Dismutase - metabolism

Fibroblasts - metabolism

Details

Title: Subtitle: N-acetyl-L-cysteine increases MnSOD activity and enhances the recruitment of quiescent human fibroblasts to the proliferation cycle during wound healing
Creators: Gaowei Mao - B180 Med Labs, Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242, USA
Monali Goswami - B180 Med Labs, Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242, USA
Amanda L Kalen - B180 Med Labs, Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242, USA
Prabhat C Goswami - B180 Med Labs, Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242, USA
Ehab H Sarsour - B180 Med Labs, Free Radical and Radiation Biology Division, Department of Radiation Oncology, University of Iowa, Iowa City, IA, 52242, USA. ehab-sarsour@uiowa.edu
Resource Type: Journal article
Publication Details: Molecular biology reports, Vol.43(1), pp.31-39
DOI: 10.1007/s11033-015-3935-1
PMID: 26671656
PMCID: PMC4822548
NLM abbreviation: Mol Biol Rep
ISSN: 0301-4851
eISSN: 1573-4978
Publisher: Netherlands
Grant note: 2R01 CA111365 / NCI NIH HHS R01 CA111365 / NCI NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 01/2016
Academic Unit: Radiation Oncology
Record Identifier: 9984047706402771

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