Journal article
N-terminomics identifies Prli42 as a membrane miniprotein conserved in Firmicutes and critical for stressosome activation in Listeria monocytogenes
Nature microbiology, Vol.2(5), pp.17005-17005
02/13/2017
DOI: 10.1038/nmicrobiol.2017.5
PMCID: PMC5802382
PMID: 28191904
Abstract
To adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. In Gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma B regulon. The stressosome is structurally well-characterized in Bacillus, but how it senses stress remains elusive. Here, we report a genome-wide N-terminomic approach in Listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniproteins, among which one, Prli42, is conserved in Firmicutes. Prli42 is membrane-anchored and interacts with orthologues of Bacillus stressosome components. We reconstituted the Listeria stressosome in vitro and visualized its supramolecular structure by electron microscopy. Analysis of a series of Prli42 mutants demonstrated that Prli42 is important for sigma B activation, bacterial growth following oxidative stress and for survival in macrophages. Taken together, our N-terminonic approach unveiled Prli42 as a long-sought link between stress and the stressosome.
Details
- Title: Subtitle
- N-terminomics identifies Prli42 as a membrane miniprotein conserved in Firmicutes and critical for stressosome activation in Listeria monocytogenes
- Creators
- Francis Impens - Interactions Bactéries-CellulesNathalie Rolhion - Interactions Bactéries-CellulesLilliana Radoshevich - Interactions Bactéries-CellulesChristophe Bécavin - Interactions Bactéries-CellulesMélodie Duval - Interactions Bactéries-CellulesJeffrey Mellin - Interactions Bactéries-CellulesFrancisco García del Portillo - Centro Nacional de Biotecnología [Madrid]M. Graciela Pucciarelli - Centro Nacional de Biotecnología [Madrid]Allison H Williams - Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell WallPascale Cossart - Interactions Bactéries-Cellules
- Resource Type
- Journal article
- Publication Details
- Nature microbiology, Vol.2(5), pp.17005-17005
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/nmicrobiol.2017.5
- PMID
- 28191904
- PMCID
- PMC5802382
- ISSN
- 2058-5276
- eISSN
- 2058-5276
- Language
- English
- Date published
- 02/13/2017
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984025415702771
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