NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates

David DeWolfe; Malika Aid; Kevin McGann; Joshua Ghofrani; Emma Geiger; Catherine Helzer; Shaily Malik; Steve Kleiboeker; Stephanie Jost; Chen Sabrina Tan

doi:10.3389/fimmu.2019.01890

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NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates

David DeWolfe, Malika Aid, Kevin McGann, Joshua Ghofrani, Emma Geiger, Catherine Helzer, Shaily Malik, Steve Kleiboeker, Stephanie Jost and Chen Sabrina Tan

Frontiers in immunology, Vol.10, pp.1890-1890

08/23/2019

DOI: 10.3389/fimmu.2019.01890

PMCID: PMC6716214

PMID: 31507586

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Abstract

Background: A previously proposed immune risk profile (IRP), based on T cell phenotype and CMV serotype, is associated with mortality in the elderly and increased infections post-kidney transplant. To evaluate if NK cells contribute to the IRP and if the IRP can be predicted by a clinical T cell functional assays, we conducted a cross sectional study in renal transplant candidates to determine the incidence of IRP and its association with specific NK cell characteristics and ImmuKnow® value. Material and Methods: Sixty five subjects were enrolled in 5 cohorts designated by age and dialysis status. We determined T and NK cell phenotypes by flow cytometry and analyzed multiple factors contributing to IRP. Results: We identified 14 IRP+ [CMV seropositivity and CD4/CD8 ratio < 1 or being in the highest quintile of CD8+ senescent (28CD–/CD57+) T cells] individuals equally divided amongst the cohorts. Multivariable linear regression revealed a distinct IRP+ group. Age and dialysis status did not predict immune senescence in kidney transplant candidates. NK cell features alone could discriminate IRP– and IRP+ patients, suggesting that NK cells significantly contribute to the overall immune status in kidney transplant candidates and that a combined T and NK cell phenotyping can provide a more detailed IRP definition. ImmuKnow® value was negatively correlated to age and significantly lower in IRP+ patients and predicts IRP when used alone or in combination with NK cell features. Conclusion: NK cells contribute to overall immune senescence in kidney transplant candidates.

BK virus

dialysis

end stage renal disease

immune risk profile

immune senescence

Immunology

kidney transplant

NK cells

Details

Title: Subtitle: NK Cells Contribute to the Immune Risk Profile in Kidney Transplant Candidates
Creators: David DeWolfe - Beth Israel Deaconess Medical Center
Malika Aid - Beth Israel Deaconess Medical Center
Kevin McGann - Beth Israel Deaconess Medical Center
Joshua Ghofrani - Beth Israel Deaconess Medical Center
Emma Geiger - Beth Israel Deaconess Medical Center
Catherine Helzer - Beth Israel Deaconess Medical Center
Shaily Malik - Beth Israel Deaconess Medical Center
Steve Kleiboeker - Viracor-Eurofins, Lee's Summit, MO, United States.
Stephanie Jost - Beth Israel Deaconess Medical Center
Chen Sabrina Tan - Beth Israel Deaconess Medical Center
Resource Type: Journal article
Publication Details: Frontiers in immunology, Vol.10, pp.1890-1890
DOI: 10.3389/fimmu.2019.01890
PMID: 31507586
PMCID: PMC6716214
NLM abbreviation: Front Immunol
ISSN: 1664-3224
eISSN: 1664-3224
Publisher: Frontiers Media S.A
Grant note: Roche Organ Transplant Research Foundation National Institute of Neurological Disorders and Stroke
Language: English
Date published: 08/23/2019
Academic Unit: Iowa Neuroscience Institute; Internal Medicine
Record Identifier: 9984366280602771

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