Journal article
NK cytokine secretion and cytotoxicity occur independently of the SLP‐76 adaptor protein
European journal of immunology, Vol.29(7), pp.2223-2232
07/1999
DOI: 10.1002/(SICI)1521-4141(199907)29:07<2223::AID-IMMU2223>3.0.CO;2-6
PMID: 10427985
Abstract
The adapter protein SLP‐76 is required for T cell development and TCR signal transduction. SLP‐76 is also expressed in NK cells, yet splenic populations of NK cells develop normally in SLP‐76‐deficient mice. We examined the effects of SLP‐76 deficiency upon cellular activation through studies of NK function in SLP‐76–/– mice. This study presents evidence that NK populations in both spleen and liver of SLP‐76–/– mice remain intact. Natural cytotoxic responses of SLP‐76–/– splenocytes proceed in a manner comparable to those of wild‐type control splenocytes. Similar to controls, SLP‐76–/– splenocytes exhibit enhanced survival and augmented cytotoxic capacity after in vitro culture with IL‐2. IL‐2‐stimulated SLP‐76–/– splenocytes also retain normal antibody‐dependent cellular cytotoxicity and the ability to secrete IFN‐γ in response to IL‐12 stimulation. These results indicate that, unlike events stimulated by TCR engagement, signaling cascades engaged by IL‐2 and IL‐12 receptors, by FcγRIIIA (which mediates antibody‐dependent cellular cytotoxicity), and by natural cytotoxicity‐associated receptors on murine NK cells can occur independently of SLP‐76.
Details
- Title: Subtitle
- NK cytokine secretion and cytotoxicity occur independently of the SLP‐76 adaptor protein
- Creators
- Erik J PetersonJames L ClementsZuhair K BallasGary A Koretzky
- Resource Type
- Journal article
- Publication Details
- European journal of immunology, Vol.29(7), pp.2223-2232
- DOI
- 10.1002/(SICI)1521-4141(199907)29:07<2223::AID-IMMU2223>3.0.CO;2-6
- PMID
- 10427985
- NLM abbreviation
- Eur J Immunol
- ISSN
- 0014-2980
- eISSN
- 1521-4141
- Publisher
- WILEY‐VCH Verlag GmbH
- Number of pages
- 10
- Language
- English
- Date published
- 07/1999
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094628502771
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