Journal article
NK1.1+ thymocytes. Adult murine CD4-, CD8- thymocytes contain an NK1.1+, CD3+, CD5hi, CD44hi, TCR-V beta 8+ subset
The Journal of immunology (1950), Vol.145(4), pp.1039-1045
08/15/1990
DOI: 10.4049/jimmunol.145.4.1039
PMID: 1696293
Abstract
CD4-, CD8- thymocytes were purified from thymi obtained from normal C57BL/6 mice. By flow cytometry analysis, 5 to 10% of these double negative (DN) thymocytes were found to express NK1.1 on their surface. The NK1.1+ DN thymocytes were demonstrated, by two-color fluorescence, to be CD3lo, CD5hi, CD44hi, J11d-, B220-, MEL 14-, IL2R- with 60% expressing TCR-V beta 8 as determined by the mAb F23.1. In contrast, splenic and peripheral blood NK cells were NK1.1+, CD3-, CD5-, TCR-V beta 8- with 40 to 60% being MEL 14+. Unlike peripheral NK cells, fresh DN thymocytes enriched for NK1.1+ cells were unable to kill YAC-1, the classical murine NK cell target. However, these cells were able to mediate anti-CD3 redirected lysis even when they were assayed immediately after purification, i.e., with no culture or stimulation. These data demonstrate that adult murine thymocytes contain NK1.1+ cells which are distinct, both by function and phenotype, from peripheral NK cells. These data also raise the issue of a possible NK/T bipotential progenitor cell.
Details
- Title: Subtitle
- NK1.1+ thymocytes. Adult murine CD4-, CD8- thymocytes contain an NK1.1+, CD3+, CD5hi, CD44hi, TCR-V beta 8+ subset
- Creators
- Zuhair K Ballas - Iowa City Veterans Administration, IAWendy Rasmussen
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.145(4), pp.1039-1045
- DOI
- 10.4049/jimmunol.145.4.1039
- PMID
- 1696293
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AI26460 / NIAID NIH HHS
- Language
- English
- Date published
- 08/15/1990
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094761302771
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