Journal article
NMDA Prevents Alcohol-Induced Neuronal Cell Death of Cerebellar Granule Cells in Culture
Alcoholism, clinical and experimental research, Vol.19(4), pp.846-853
Received for publication August 18, 1994; accepted January 20, 1995
08/1995
DOI: 10.1111/j.1530-0277.1995.tb00957.x
PMID: 7485829
Abstract
Neuronal cell loss is one of the most debilitating effects of alcohol exposure during development of the nervous system. In this study, primary cultures of neuronal cells (cerebellar granule cells) were used to examine mechanisms of alcohol-induced neuronal cell death. Previously, we established that (Pantazis et al., Alcohol Clin Exp Res 17:1014-1021, 1993): (1) alcohol exposure caused neuronal cell death in cultures of cerebellar granule cells and this cell loss was both time-dependent and dose-dependent; and (2) the vulnerability of cerebellar granule cells to alcohol-induced loss changed with the length of time the cells were in culture before initiating alcohol exposure-that is, younger cultures (1 day in vitro) were much more susceptible to alcohol-induced neuronal cell death than older cultures (4 or 7 days in vitro). The primary goal of the present study was to examine the potential role of the NMDA receptor in alcohol-induced death of cerebellar granule cells in culture. Experiments were performed to test the hypothesis that the alcohol-induced death of cerebellar granule cells can be prevented or reduced by NMDA treatment. Our results indicate that stimulation of the NMDA receptor has a neuroprotective effect and can significantly reduce the alcohol-induced neuronal cell death of newly established cerebellar granule cell cultures. This neuroprotective effect of NMDA is blocked by 2-amino-5-phosphonovalerate, a competitive inhibitor of the NMDA receptor, confirming that this neuroprotective effect is mediated via the NMDA receptor. This is the first report that alcohol's neurotoxic effect can be ameliorated by activation of the NMDA receptor.
Details
- Title: Subtitle
- NMDA Prevents Alcohol-Induced Neuronal Cell Death of Cerebellar Granule Cells in Culture
- Creators
- Nicholas J Pantazis - University of IowaDouglas P Dohrman - University of California, San FranciscoJia Luo - University of IowaJennifer D Thomas - University of IowaCharles R Goodlett - Purdue University West LafayetteJames R West - Texas A&M University
- Resource Type
- Journal article
- Publication Details
- Alcoholism, clinical and experimental research, Vol.19(4), pp.846-853
- Edition
- Received for publication August 18, 1994; accepted January 20, 1995
- DOI
- 10.1111/j.1530-0277.1995.tb00957.x
- PMID
- 7485829
- NLM abbreviation
- Alcohol Clin Exp Res
- ISSN
- 0145-6008
- eISSN
- 1530-0277
- Publisher
- Blackwell Publishing Ltd
- Number of pages
- 8
- Language
- English
- Date published
- 08/1995
- Academic Unit
- Anatomy and Cell Biology; Pathology
- Record Identifier
- 9984186656202771
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