Journal article
NOX2 Protects against Prolonged Inflammation, Lung Injury, and Mortality following Systemic Insults
Journal of innate immunity, Vol.5(6), pp.565-580
11/2013
DOI: 10.1159/000347212
PMCID: PMC6741530
PMID: 23635512
Abstract
The systemic inflammatory response syndrome (SIRS) is a clinical condition occurring in intensive care unit patients as a consequence of both infectious and noninfectious insults. The mechanisms underlying resolution of SIRS are not well characterized. NOX2 (NADPH oxidase 2)-derived reactive oxygen species are critical for killing of certain pathogens by polymorphonuclear leukocytes (PMN). Patients with chronic granulomatous disease who lack functional NOX2 are not only prone to serious infections, they also exhibit chronic inflammatory conditions, suggesting a local anti-inflammatory role for NOX2. We hypothesized that NOX2 is required for the resolution of sterile systemic inflammation. Using a murine model of sterile generalized inflammation, we observed dramatically increased mortality of gp91phox-/y (NOX2-deficient) as compared to wild-type (WT) mice. Both genotypes developed robust SIRS with hypothermia, hypotension, and leukopenia; however, WT mice recovered within 48 h whereas NOX2-deficient mice did not. Although both groups displayed rapid peritoneal PMN recruitment, the recruited NOX2-deficient PMN demonstrated an enhanced inflammatory phenotype. Moreover, NOX2-deficient mice exhibited a hemorrhagic inflammatory response in the lungs with rapid and persistent recruitment of neutrophils to the alveolar space, whereas WT mice had minimal lung pathology. Several proinflammatory cytokines remained elevated in NOX2-deficient mice. The persistent inflammatory environment observed in NOX2-deficient mice resulted from continued peritoneal chemokine secretion and not delayed apoptosis of PMN. These data suggest a requirement for NOX2 in the resolution of systemic inflammation.
Details
- Title: Subtitle
- NOX2 Protects against Prolonged Inflammation, Lung Injury, and Mortality following Systemic Insults
- Creators
- Laura C WhitmoreBrieanna M HilkinKelli L GossErin M WahleTarah T ColaizyPaola M BoggiattoSteven M VargaFrancis J MillerJessica G Moreland
- Resource Type
- Journal article
- Publication Details
- Journal of innate immunity, Vol.5(6), pp.565-580
- DOI
- 10.1159/000347212
- PMID
- 23635512
- PMCID
- PMC6741530
- ISSN
- 1662-811X
- eISSN
- 1662-8128
- Number of pages
- 16
- Language
- English
- Date published
- 11/2013
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Neonatology; Internal Medicine
- Record Identifier
- 9984083284602771
Metrics
51 Record Views