Journal article
NOX5 variants are functionally active in endothelial cells
Free radical biology & medicine, Vol.42(4), pp.446-459
2007
DOI: 10.1016/j.freeradbiomed.2006.10.054
PMID: 17275676
Abstract
NADPH oxidases have been identified as sources of reactive oxygen species (ROS) in vascular cells. In addition to the initially described enzyme containing gp91phox (NOX2), several homologues to NOX2 have been identified. Whereas NOX1, NOX2, and NOX4 are expressed in endothelial cells, a functional role of NOX5 containing additional N-terminal calcium-binding domains of varying sequences has not been reported in these cells. NOX5 protein was found in the endoplasmic reticulum of human microvascular endothelial cells (HMEC-1) and in the vascular wall. HMEC-1 cells expressed NOX5β and NOX5δ as well as a variant lacking calcium-binding domains (NOX5S). NOX5β and NOX5S increased basal ROS levels. Ionomycin exclusively enhanced NOX5β-mediated ROS production. Although p22phox, when overexpressed, interacted with both NOX5 proteins, it was not essential for NOX5-mediated ROS production. NOX5 proteins stimulated endothelial cell proliferation and the formation of capillary-like structures whereas depletion of NOX5 by siRNA prevented these responses to thrombin. These data show that endothelial cells express different NOX5 variants including NOX5S lacking calcium-binding domains. NOX5 proteins are functional, promoting endothelial ROS production, proliferation, and the formation of capillary-like structures and contribute to the endothelial response to thrombin. These findings suggest that NOX5 variants play a novel role in controlling ROS-dependent processes in the vasculature.
Details
- Title: Subtitle
- NOX5 variants are functionally active in endothelial cells
- Creators
- Rachida S. BelAiba - Deutsches Herzzentrum MünchenTalija Djordjevic - Deutsches Herzzentrum MünchenAndreas Petry - Deutsches Herzzentrum MünchenKerstin Diemer - Deutsches Herzzentrum MünchenSteve Bonello - Deutsches Herzzentrum MünchenBotond Banfi - University of IowaJohn Hess - Deutsches Herzzentrum MünchenAlexej Pogrebniak - Deutsches Herzzentrum MünchenChristian Bickel - Deutsches Herzzentrum MünchenAgnes Görlach - Deutsches Herzzentrum München
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.42(4), pp.446-459
- DOI
- 10.1016/j.freeradbiomed.2006.10.054
- PMID
- 17275676
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2007
- Academic Unit
- Anatomy and Cell Biology; Otolaryngology; Internal Medicine
- Record Identifier
- 9984284459302771
Metrics
11 Record Views