Journal article
NUCLEOTIDE-SEQUENCE OF YEAST GDH1 ENCODING NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE-DEPENDENT GLUTAMATE-DEHYDROGENASE
The Journal of biological chemistry, Vol.260(14), pp.8502-8508
01/01/1985
DOI: 10.1016/S0021-9258(17)39500-5
PMID: 2989290
Abstract
The yeast GDH1 gene encodes NADP-dependent glutamate dehydrogenase. This gene was isolated by complementation of an Escherichia coli glutamate auxotroph. NADP-dependent glutamate dehydrogenase was overproduced 6-10-fold in Saccharomyces cerevisiae bearing GDH1 on a multicopy plasmid. The nucleotide sequence of the 1362-base pair coding region and 5' and 3' flanking sequences were determined. Transcription start sites were located by S1 nuclease mapping. Regulation of GDH1 was not maintained when the gene was present on a multicopy plasmid. Protein secondary structure predictions identified a region with potential to form the dinucleotide-binding domain. The amino acid sequences of the yeast and Neurospora crassa enzymes are 63% conserved. Unlike the N. crassa gene, yeast GDH1 has no introns.
Details
- Title: Subtitle
- NUCLEOTIDE-SEQUENCE OF YEAST GDH1 ENCODING NICOTINAMIDE ADENINE-DINUCLEOTIDE PHOSPHATE-DEPENDENT GLUTAMATE-DEHYDROGENASE
- Creators
- W Scott MoyeNaoki AmuroJ. K. Mohana RaoHoward Zalkin
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.260(14), pp.8502-8508
- DOI
- 10.1016/S0021-9258(17)39500-5
- PMID
- 2989290
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- Amer Soc Biochemistry Molecular Biology Inc
- Number of pages
- 7
- Grant note
- GM07211; GM24658 / NIGMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R37GM024658 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Language
- English
- Date published
- 01/01/1985
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297609202771
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