Journal article
Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
Journal of virology, Vol.91(1), e01830-16
01/01/2017
DOI: 10.1128/JVI.01830-16
PMCID: PMC5165213
PMID: 27795436
Abstract
HIV-1 latency is characterized by reversible silencing of viral transcription driven by the long terminal repeat (LTR) promoter of HIV-1. Cellular and viral factors regulating LTR activity contribute to HIV-1 latency, and certain repressive cellular factors modulate viral transcription silencing. Nef-associated factor 1 (Naf1) is a host nucleocytoplasmic shuttling protein that regulates multiple cellular signaling pathways and HIV-1 production. We recently reported that nuclear Naf1 promoted nuclear export of unspliced HIV-1 gag mRNA, leading to increased Gag production. Here we demonstrate new functions of Naf1 in regulating HIV-1 persistence. We found that Naf1 contributes to the maintenance of HIV-1 latency by inhibiting LTR-driven HIV-1 gene transcription in a nuclear factor kappa B-dependent manner. Interestingly, Naf1 knockdown significantly enhanced viral reactivation in both latently HIV-1-infected Jurkat T cells and primary central memory CD4
Details
- Title: Subtitle
- Naf1 Regulates HIV-1 Latency by Suppressing Viral Promoter-Driven Gene Expression in Primary CD4+ T Cells
- Creators
- Chuan Li - Guangzhou Women and Children's Medical Center, Guangzhou, ChinaHai-Bo Wang - CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaWen-Dong Kuang - CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaXiao-Xin Ren - CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaShu-Ting Song - CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, ChinaHuan-Zhang Zhu - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, ChinaQiang Li - Key Laboratory of Prevention and Treatment with Traditional Chinese Medicine of Viral Infectious Disease, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, ChinaLi-Ran Xu - Key Laboratory of Prevention and Treatment with Traditional Chinese Medicine of Viral Infectious Disease, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, ChinaHui-Jun Guo - First Affiliated Hospital of Henan University of Traditional Chinese MedicineLi Wu - Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USAJian-Hua Wang - Guangzhou Women and Children's Medical Center, Guangzhou, China
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.91(1), e01830-16
- Publisher
- United States
- DOI
- 10.1128/JVI.01830-16
- PMID
- 27795436
- PMCID
- PMC5165213
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Grant note
- R01 AI104483 / NIAID NIH HHS R21 CA181997 / NCI NIH HHS R56 AI127667 / NIAID NIH HHS R01 AI120209 / NIAID NIH HHS
- Language
- English
- Date published
- 01/01/2017
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984001118002771
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