Journal article
Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors
Analytical chemistry (Washington), Vol.84(15), pp.6341-6345
08/07/2012
DOI: 10.1021/ac301202q
PMID: 22741519
Abstract
The ability of nanoassisted laser desorption-ionization mass spectrometry (NALDI-MS) imaging to provide selective chemical monitoring with proper spatial distribution of lipid profiles from tumor tissues after plate imprinting has been tested. NALDI-MS imaging identified and mapped several potential lipid biomarkers in a murine model of melanoma tumor (inoculation of B16/F10 cells). It also confirmed that the in vivo treatment of tumor bearing mice with synthetic supplement containing phosphoethanolamine (PHO-S) promoted an accentuated decrease in relative abundance of the tumor biomarkers. NALDI-MS imaging is a matrix-free LDI protocol based on the selective imprinting of lipids in the NALDI plate followed by the removal of the tissue. It therefore provides good quality and selective chemical images with preservation of spatial distribution and less interference from tissue material. The test case described herein illustrates the potential of chemically selective NALDI-MS imaging for biomarker discovery.
Details
- Title: Subtitle
- Nanoassisted Laser Desorption-Ionization-MS Imaging of Tumors
- Creators
- Alessandra Tata - Universidade Estadual de Campinas (UNICAMP)Anna Maria A. P. Fernandes - Universidade Estadual de Campinas (UNICAMP)Vanessa G. Santos - Universidade Estadual de Campinas (UNICAMP)Rosana M. Alberici - Universidade Estadual de Campinas (UNICAMP)Dioneia Araldi - Universidade Estadual de Campinas (UNICAMP)Carlos A. Parada - Universidade Estadual de Campinas (UNICAMP)Wellington Braguini - Sch Nursing, Dept Maternal & Child Nursing & Publ Hlth, Ribeirao Preto, SP, BrazilLuciana Veronez - Clinics Hospital of Ribeirão PretoGabriela Silva Bisson - Clinics Hospital of Ribeirão PretoFelippe H. Z. Reis - Universidade de São PauloLuciane C. Alberici - Universidade de São PauloMarcos N. Eberlin - Universidade Estadual de Campinas (UNICAMP)
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.84(15), pp.6341-6345
- DOI
- 10.1021/ac301202q
- PMID
- 22741519
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Publisher
- Amer Chemical Soc
- Number of pages
- 5
- Grant note
- CNPq; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) CAPES; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) FAPESP; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
- Language
- English
- Date published
- 08/07/2012
- Academic Unit
- Neuroscience and Pharmacology
- Record Identifier
- 9985178669302771
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