Nanoemulsion Thermoreversible Pluronic F127-Based Hydrogel Containing Hyptis pectinata (Lamiaceae) Leaf Essential Oil Produced a Lasting Anti-hyperalgesic Effect in Chronic Noninflammatory Widespread Pain in Mice

Lucindo J Quintans-Júnior; Renan G Brito; Jullyana S S Quintans; Priscila L Santos; Zaine T Camargo; Péricles A Barreto; Maria F Arrigoni-Blank; Waldecy Lucca-Júnior; Luciana Scotti; Marcus T Scotti; Sandra J Kolker; Kathleen A Sluka

doi:10.1007/s12035-017-0438-1

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Nanoemulsion Thermoreversible Pluronic F127-Based Hydrogel Containing Hyptis pectinata (Lamiaceae) Leaf Essential Oil Produced a Lasting Anti-hyperalgesic Effect in Chronic Noninflammatory Widespread Pain in Mice

Journal article

Peer reviewed

Nanoemulsion Thermoreversible Pluronic F127-Based Hydrogel Containing Hyptis pectinata (Lamiaceae) Leaf Essential Oil Produced a Lasting Anti-hyperalgesic Effect in Chronic Noninflammatory Widespread Pain in Mice

Lucindo J Quintans-Júnior, Renan G Brito, Jullyana S S Quintans, Priscila L Santos, Zaine T Camargo, Péricles A Barreto, Maria F Arrigoni-Blank, Waldecy Lucca-Júnior, Luciana Scotti, Marcus T Scotti, …

Molecular neurobiology, Vol.55(2), pp.1665-1675

02/2018

DOI: 10.1007/s12035-017-0438-1

PMID: 28194646

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Abstract

We evaluated if a nanostructured thermoreversible Pluronic F127-based hydrogel incorporated with Hyptis pectinata leaf essential oil (NE-EOH) produces a long-lasting anti-hyperalgesic effect on chronic muscle pain in an animal model. We induced chronic muscle pain by injecting the gastrocnemius with saline injections. Paw and muscle withdrawal thresholds and motor performance were evaluated after treatment and compared with morphine, diazepam, or vehicle. Naloxone and methysergide administration tested the involvement of opioid and serotonin receptors, respectively. Sites of action in the central nervous system for the NE-EOH were examined by measuring substance P (SP) levels in the spinal cord and Fos protein in the brainstem. NE-EOH increased paw and muscle withdrawal thresholds when compared with vehicle but had no effect on motor function. This analgesic effect was reversed by both naloxone and methysergide. NE-EOH decreased elevated substance P levels and reduced Fos-labeled neurons in the spinal cord and increased the number of Fos-labeled neurons in the periaqueductal gray (PAG), nucleus raphe magnus (NRM), and locus coeruleus (LC). NE-EOH was shown to produce a lasting anti-hyperalgesic effect. It uses opioid and serotonin receptors, activates brainstem inhibitory pathways, and reduces the release of excitatory neurotransmitters in the spinal cord and is a substance with potential to be used in the treatment of noninflammatory pain conditions. Graphical Abstract.

Analgesics - pharmacology

Analgesics - therapeutic use

Chronic Pain - drug therapy

Spinal Cord - drug effects

Spinal Cord - metabolism

Plant Extracts - pharmacology

Periaqueductal Gray - drug effects

Proto-Oncogene Proteins c-fos - metabolism

Male

Oils, Volatile - pharmacology

Lamiaceae

Periaqueductal Gray - metabolism

Chronic Pain - metabolism

Oils, Volatile - therapeutic use

Animals

Hydrogel, Polyethylene Glycol Dimethacrylate

Substance P - metabolism

Mice

Pain Measurement

Plant Extracts - therapeutic use

Disease Models, Animal

Details

Title: Subtitle: Nanoemulsion Thermoreversible Pluronic F127-Based Hydrogel Containing Hyptis pectinata (Lamiaceae) Leaf Essential Oil Produced a Lasting Anti-hyperalgesic Effect in Chronic Noninflammatory Widespread Pain in Mice
Creators: Lucindo J Quintans-Júnior - Department of Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa (UI), Iowa City, IA, USA. lucindojr@gmail.com
Renan G Brito - Department of Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa (UI), Iowa City, IA, USA
Jullyana S S Quintans - Department of Physiology (DFS), Laboratory of Neuroscience and Pharmacological Assays (LANEF), Federal University of Sergipe (UFS), Av. Marechal Rondom, s/n, São Cristóvão, SE, Brazil
Priscila L Santos - Department of Physiology (DFS), Laboratory of Neuroscience and Pharmacological Assays (LANEF), Federal University of Sergipe (UFS), Av. Marechal Rondom, s/n, São Cristóvão, SE, Brazil
Zaine T Camargo - Department of Chemistry, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil
Péricles A Barreto - Department of Chemistry, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil
Maria F Arrigoni-Blank - Department of Agronomy, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil
Waldecy Lucca-Júnior - Department of Morphology, Federal University of Sergipe (UFS), São Cristóvão, SE, Brazil
Luciana Scotti - Department of Chemistry, Federal University of Paraíba (UFPB), João Pessoa, PB, Brazil
Marcus T Scotti - Department of Chemistry, Federal University of Paraíba (UFPB), João Pessoa, PB, Brazil
Sandra J Kolker - Department of Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa (UI), Iowa City, IA, USA
Kathleen A Sluka - Department of Physical Therapy and Rehabilitation Science, Carver College of Medicine, University of Iowa (UI), Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Molecular neurobiology, Vol.55(2), pp.1665-1675
Publisher: United States
DOI: 10.1007/s12035-017-0438-1
PMID: 28194646
ISSN: 0893-7648
eISSN: 1559-1182
Grant note: 441969/2014-3 / CNPq/Brazil
Language: English
Date published: 02/2018
Academic Unit: Iowa Neuroscience Institute; Nursing; Anesthesia; Physical Therapy and Rehabilitation Science; Neuroscience and Pharmacology
Record Identifier: 9984040288202771

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