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Nanoencapsulating living biological cells using electrostatic layer-by-layer self-assembly: platelets as a model
Journal article   Open access   Peer reviewed

Nanoencapsulating living biological cells using electrostatic layer-by-layer self-assembly: platelets as a model

Qinghe Zhao, Hongshuai Li and Bingyun Li
Journal of materials research, Vol.26(2), pp.347-351
01/01/2011
DOI: 10.1557/jmr.2010.23
PMCID: PMC3045201
PMID: 21359101
url
https://www.ncbi.nlm.nih.gov/pmc/articles/3045201View
Open Access

Abstract

In the literature, a few biological cells have been used as templates to form microcapsules of a variety of shapes and sizes. In this study, we proved the concept that living cells like platelets can be encapsulated with polyelectrolytes using electrostatic layer-by-layer self-assembly (LBL), and, most importantly, the encapsulation process did not induce activation of the platelets. Glycol-chitosan and poly-L-glutamic acid were electrostatically deposited onto platelets, and the encapsulation was confirmed using confocal laser scanning microscopy and scanning electron microscopy. Transmission electron microscopy observation further confirmed that the encapsulation process was mild and the activation of platelets was negligible. The encapsulation of living biological cells like platelets can serve as a model system in a wide range of biomedical applications including local and sustained drug delivery, immune protection of artificial tissues, and versatile artificial blood.
cell encapsulation Electrostatic layer-by-layer self-assembly platelet polyelectrolyte

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