Journal article
Nanoparticle-mediated conversion of primary human astrocytes into neurons and oligodendrocytes
Biomaterials science, Vol.4(7), pp.1100-1112
06/21/2016
DOI: 10.1039/c6bm00140h
PMID: 27328202
Abstract
Central nervous system (CNS) diseases and injuries are accompanied by reactive gliosis and scarring involving the activation and proliferation of astrocytes to form hypertrophic and dense structures, which present a significant barrier to neural regeneration. Engineering astrocytes to functional neurons or oligo-dendrocytes may constitute a novel therapeutic strategy for CNS diseases and injuries. Such direct cellular programming has been successfully demonstrated using viral vectors via the transduction of transcriptional factors, such as Sox2, which could program resident astrocytes into neurons in the adult brain and spinal cord, albeit the efficiency was low. Here we report a non-viral nanoparticle-based transfection method to deliver Sox2 or Olig2 into primary human astrocytes and demonstrate the effective conversion of the astrocytes into neurons and oligodendrocyte progenitors following the transgene expression of Sox2 and Olig2, respectively. This approach is highly translatable for engineering astrocytes to repair injured CNS tissues.
Details
- Title: Subtitle
- Nanoparticle-mediated conversion of primary human astrocytes into neurons and oligodendrocytes
- Creators
- Xiaowei Li - Johns Hopkins UniversityKristen Kozielski - Johns Hopkins UniversityYu-Hao Cheng - Johns Hopkins Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21287 USAHuanhuan Liu - Johns Hopkins UniversityCamila Gadens Zamboni - Johns Hopkins UniversityJordan Green - Johns Hopkins UniversityHai-Quan Mao - Johns Hopkins Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21287 USA
- Resource Type
- Journal article
- Publication Details
- Biomaterials science, Vol.4(7), pp.1100-1112
- DOI
- 10.1039/c6bm00140h
- PMID
- 27328202
- NLM abbreviation
- Biomater Sci
- ISSN
- 2047-4830
- eISSN
- 2047-4849
- Publisher
- Royal Soc Chemistry
- Number of pages
- 13
- Grant note
- R21NS085714 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) 2013MSCRF-00042169 / Maryland Stem Cell Research Fund P30EY001765 / NATIONAL EYE INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Eye Institute (NEI) R01EB001672 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) R21NS085714-01A1 / U.S. National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) F31CA196163 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 1R01EB01672 / National Institute of Biomedical Imaging and Bioengineering; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
- Language
- English
- Date published
- 06/21/2016
- Academic Unit
- Radiology
- Record Identifier
- 9985179850902771
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