Journal article
Nanoparticulated heat-stable (STa) and heat-labile B subunit (LTB) recombinant toxin improves vaccine protection against enterotoxigenic Escherichia coli challenge in mouse
Journal of bioscience and bioengineering, Vol.115(2), pp.147-153
02/2013
DOI: 10.1016/j.jbiosc.2012.09.009
PMID: 23040995
Abstract
Enterotoxigenic Escherichia coli (ETEC) remains a major cause of diarrheic disease in developing areas, for which there is no effective vaccine available. In this study, we genetically engineered a recombinant heat-stable enterotoxin (STa) coupled to the subunit B of heat-labile enterotoxin (LTB). This fusion protein, STa-LTB, possesses a single amino acid substitution at position 14 of STa. Our data demonstrates that the enterotoxicity of STa in STa-LTB was dramatically reduced. A gelatin nanovaccine candidate was prepared using the purified STa-LTB fusion protein characterized with an entrapment efficiency of 84.88 ± 6.37% and smooth spheres size ranges of 80–200 nm. Antigen-specific antibody responses against STa-LTB and STa in the sera and the intestinal mucus respectively were used to test the immunogenicity of the nanovaccine. This vaccine was further screened in mice by its ability to elicit neutralizing antibodies against STa and protect animals from the challenge with ETEC in mice. The STa-LTB nanoparticles delivered demonstrated a capacity to induce significantly higher and long-lasting antibody responses and increased immune protection against ETEC challenge relative to the control STa-LTB vaccine absorbed in conventional aluminum hydrate salt (p < 0.01). These results warrant the further studies of the development of a novel nanoparticulate vaccine as a broad-spectrum vaccine against ETEC infection.
Details
- Title: Subtitle
- Nanoparticulated heat-stable (STa) and heat-labile B subunit (LTB) recombinant toxin improves vaccine protection against enterotoxigenic Escherichia coli challenge in mouse
- Creators
- Guangcun Deng - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR ChinaJin Zeng - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR ChinaMinjie Jian - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR ChinaWenmiao Liu - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR ChinaZhong Zhang - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR ChinaXiaoming Liu - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR ChinaYujiong Wang - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China, 539 W. Helanshan Road, Yinchuan, Ningxia 750021, PR China
- Resource Type
- Journal article
- Publication Details
- Journal of bioscience and bioengineering, Vol.115(2), pp.147-153
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jbiosc.2012.09.009
- PMID
- 23040995
- ISSN
- 1389-1723
- eISSN
- 1347-4421
- Grant note
- name: National Research Program of China, award: 2012BAD12B07-03; DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 30860207, 31060335; name: Ningxia Natural Science Foundation, award: NZ1039
- Language
- English
- Date published
- 02/2013
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288800902771
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