Journal article
Narrow-Spectrum Inhibitors of Campylobacter jejuni Flagellar Expression and Growth
Antimicrobial agents and chemotherapy, Vol.59(7), pp.3880-3886
07/01/2015
DOI: 10.1128/AAC.04926-14
PMCID: PMC4468704
PMID: 25870073
Abstract
Campylobacter jejuni is a major cause of food-borne illness due to its ability to reside within the gastrointestinal tracts of chickens. Multiple studies have identified the flagella of C. jejuni as a major determinant of chicken colonization. An inhibitor screen of approximately 147,000 small molecules was performed to identify compounds that are able to inhibit flagellar expression in a reporter strain of C. jejuni. Several compounds that modestly inhibited motility of wild-type C. jejuni in standard assays were identified, as were a number of small molecules that robustly inhibited C. jejuni growth, in vitro. Examination of similar bacterial screens found that many of these small molecules inhibited only the growth of C. jejuni. Follow-up assays demonstrated inhibition of other strains of C. jejuni and Campylobacter coli but no inhibition of the closely related Helicobacter pylori. The compounds were determined to be bacteriostatic and nontoxic to eukaryotic cells. Preliminary results from a day-of-hatch chick model of colonization suggest that at least one of the compounds demonstrates promise for reducing Campylobacter colonization loads in vivo, although further medicinal chemistry may be required to enhance bioavailability.
Details
- Title: Subtitle
- Narrow-Spectrum Inhibitors of Campylobacter jejuni Flagellar Expression and Growth
- Creators
- Jeremiah G Johnson - University of Michigan–Ann ArborCaroline Yuhas - University of Michigan–Ann ArborThomas J McQuade - University of Michigan–Ann ArborMartha J Larsen - University of Michigan–Ann ArborVictor J DiRita - University of Michigan–Ann Arbor
- Resource Type
- Journal article
- Publication Details
- Antimicrobial agents and chemotherapy, Vol.59(7), pp.3880-3886
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/AAC.04926-14
- PMID
- 25870073
- PMCID
- PMC4468704
- ISSN
- 0066-4804
- eISSN
- 1098-6596
- Number of pages
- 7
- Alternative title
- Small-Molecule Inhibitors of , Johnson et al
- Language
- English
- Date published
- 07/01/2015
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696582302771
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