Journal article
Nasal ciliated cells are primary targets for SARS-CoV-2 replication in the early stage of COVID-19
The Journal of clinical investigation, Vol.131(13), pp.1-14
07/01/2021
DOI: 10.1172/JCI148517
PMID: 34003804
Abstract
The upper respiratory tract is compromised in the early period of COVID-19, but SARS-CoV-2 tropism at the cellular level is not fully defined. Unlike recent single-cell RNA-Seq analyses indicating uniformly low mRNA expression of SARS-CoV-2 entry–related host molecules in all nasal epithelial cells, we show that the protein levels are relatively high and that their localizations are restricted to the apical side of multiciliated epithelial cells. In addition, we provide evidence in patients with COVID-19 that SARS-CoV-2 is massively detected and replicated within the multiciliated cells. We observed these findings during the early stage of COVID-19, when infected ciliated cells were rapidly replaced by differentiating precursor cells. Moreover, our analyses revealed that SARS-CoV-2 cellular tropism was restricted to the nasal ciliated versus oral squamous epithelium. These results imply that targeting ciliated cells of the nasal epithelium during the early stage of COVID-19 could be an ideal strategy to prevent SARS-CoV-2 propagation.
Details
- Title: Subtitle
- Nasal ciliated cells are primary targets for SARS-CoV-2 replication in the early stage of COVID-19
- Creators
- Ji Hoon Ahn - Korea Advanced Institute of Science and TechnologyJungMo Kim - Institute for Basic ScienceSeon Pyo Hong - Institute for Basic ScienceSung Yong Choi - Eulji UniversityMyung Jin Yang - Korea Advanced Institute of Science and TechnologyYoung Seok Ju - Korea Advanced Institute of Science and TechnologyYoung Tae Kim - Seoul National University HospitalHo Min Kim - Korea Advanced Institute of Science and TechnologyMD Tazikur Rahman - Jeonbuk National University HospitalMan Ki Chung - Samsung Medical CenterSang Duk Hong - Samsung Medical CenterHosung Bae - Institute for Basic ScienceChang-Seop LeeGou Young Koh - Korea Advanced Institute of Science and Technology
- Resource Type
- Journal article
- Publication Details
- The Journal of clinical investigation, Vol.131(13), pp.1-14
- DOI
- 10.1172/JCI148517
- PMID
- 34003804
- ISSN
- 0021-9738
- eISSN
- 1558-8238
- Publisher
- American Society for Clinical Investigation
- Grant note
- IBS-R025-D1 to G.Y.K,NRF-2018R1D1A1B07044974 to S.Y.C / ;
- Language
- English
- Date published
- 07/01/2021
- Academic Unit
- Internal Medicine
- Record Identifier
- 9985217131502771
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