Journal article
Native adiponectin plays a role in the adipocyte-mediated conversion of fibroblasts to myofibroblasts
Journal of the Royal Society interface, Vol.20(202), 20230004
05/2023
DOI: 10.1098/rsif.2023.0004
PMCID: PMC10154927
PMID: 37132228
Appears in UI Libraries Support Open Access
Abstract
Adipocytes regulate tissues through production of adipokines that can act both locally and systemically. Adipocytes also have been found to play a critical role in regulating the healing process. To better understand this role, we developed a three-dimensional human adipocyte spheroid system that has an adipokine profile similar to
adipose tissues. Previously, we found that conditioned medium from these spheroids induces human dermal fibroblast conversion into highly contractile, collagen-producing myofibroblasts through a transforming growth factor beta-1 (TGF-β1) independent pathway. Here, we sought to identify how mature adipocytes signal to dermal fibroblasts through adipokines to induce myofibroblast conversion. By using molecular weight fractionation, heat inactivation and lipid depletion, we determined mature adipocytes secrete a factor that is 30-100 kDa, heat labile and lipid associated that induces myofibroblast conversion. We also show that the depletion of the adipokine adiponectin, which fits those physico-chemical parameters, eliminates the ability of adipocyte-conditioned media to induce fibroblast to myofibroblast conversion. Interestingly, native adiponectin secreted by cultured adipocytes consistently elicited a stronger level of α-smooth muscle actin expression than exogenously added adiponectin. Thus, adiponectin secreted by mature adipocytes induces fibroblast to myofibroblast conversion and may lead to a phenotype of myofibroblasts distinct from TGF-β1-induced myofibroblasts.
Details
- Title: Subtitle
- Native adiponectin plays a role in the adipocyte-mediated conversion of fibroblasts to myofibroblasts
- Creators
- Mariam Y El-Hattab - University of IowaNoah Sinclair - University of IowaJesse N Liszewski - University of IowaMichael V Schrodt - University of IowaJacob Herrmann - University of IowaAloysius J Klingelhutz - University of IowaEdward A Sander - University of IowaJames A Ankrum - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of the Royal Society interface, Vol.20(202), 20230004
- DOI
- 10.1098/rsif.2023.0004
- PMID
- 37132228
- PMCID
- PMC10154927
- NLM abbreviation
- J R Soc Interface
- ISSN
- 1742-5662
- eISSN
- 1742-5662
- Publisher
- The Royal Society
- Grant note
- DOI: 10.13039/100000050, name: National Heart, Lung, and Blood Institute, award: T32HL144461; name: National Cancer Institute, award: P30CA086862; DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: P42 ES013661; DOI: 10.13039/100001583, name: Diabetes Action Research and Education Foundation; DOI: 10.13039/100000069, name: National Institute of Arthritis and Musculoskeletal and Skin Diseases, award: AR075137
- Language
- English
- Date published
- 05/2023
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Microbiology and Immunology; Iowa Technology Institute; Orthopedics and Rehabilitation; Radiation Oncology; Craniofacial Anomalies Research Center; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Chemical and Biochemical Engineering; Iowa Superfund Research Program
- Record Identifier
- 9984400758602771
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