Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells

Rene Garcia-Contreras; Patricia Alejandra Chavez-Granados; Carlos Alberto Jurado; Benjamin Aranda-Herrera; Kelvin Ian Afrashtehfar; Hamid Nurrohman

doi:10.3390/biomimetics8010075

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Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells

Journal article

Open access

Peer reviewed

Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells

Rene Garcia-Contreras, Patricia Alejandra Chavez-Granados, Carlos Alberto Jurado, Benjamin Aranda-Herrera, Kelvin Ian Afrashtehfar and Hamid Nurrohman

Biomimetics (Basel, Switzerland), Vol.8(1), 75

02/10/2023

DOI: 10.3390/biomimetics8010075

PMCID: PMC9944806

PMID: 36810406

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Abstract

The (-)-Epigallocatechin-gallate (EGCG) metabolite is a natural polyphenol derived from green tea and is associated with antioxidant, biocompatible, and anti-inflammatory effects. OBJECTIVE To evaluate the effects of EGCG to promote the odontoblast-like cells differentiated from human dental pulp stem cells (hDPSCs); the antimicrobial effects on Escherichia coli, Streptococcus mutans, and Staphylococcus aureus; and improve the adhesion on enamel and dentin by shear bond strength (SBS) and the adhesive remnant index (ARI). MATERIAL AND METHODS hDSPCs were isolated from pulp tissue and immunologically characterized. EEGC dose-response viability was calculated by MTT assay. Odontoblast-like cells were differentiated from hDPSCs and tested for mineral deposition activity by alizarin red, Von Kossa, and collagen/vimentin staining. Antimicrobial assays were performed in the microdilution test. Demineralization of enamel and dentin in teeth was performed, and the adhesion was conducted by incorporating EGCG in an adhesive system and testing with SBS-ARI. The data were analyzed with normalized Shapiro-Wilks test and ANOVA post hoc Tukey test. RESULTS The hDPSCs were positive to CD105, CD90, and vimentin and negative to CD34. EGCG (3.12 µg/mL) accelerated the differentiation of odontoblast-like cells. Streptococcus mutans exhibited the highest susceptibility < Staphylococcus aureus < Escherichia coli. EGCG increased (p < 0.05) the dentin adhesion, and cohesive failure was the most frequent. CONCLUSION (-)-Epigallocatechin-gallate is nontoxic, promotes differentiation into odontoblast-like cells, possesses an antibacterial effect, and increases dentin adhesion.

Biomedical Engineering

Bioengineering

Biotechnology

Biochemistry

polyphenols

dentin repair

Molecular Medicine

610 Medicine & health

biomodification

resin–dentin bond

Biomaterials

Details

Title: Subtitle: Natural Bioactive Epigallocatechin-Gallate Promote Bond Strength and Differentiation of Odontoblast-like Cells
Creators: Rene Garcia-Contreras
Patricia Alejandra Chavez-Granados
Carlos Alberto Jurado
Benjamin Aranda-Herrera
Kelvin Ian Afrashtehfar
Hamid Nurrohman
Resource Type: Journal article
Publication Details: Biomimetics (Basel, Switzerland), Vol.8(1), 75
DOI: 10.3390/biomimetics8010075
PMID: 36810406
PMCID: PMC9944806
NLM abbreviation: Biomimetics (Basel)
eISSN: 2313-7673
Publisher: MDPI AG
Grant note: name: UNAM-DGAPA-PAPIME, award: PE203622; name: PAPIIT, award: IT200922
Language: English
Date published: 02/10/2023
Academic Unit: Prosthodontics
Record Identifier: 9984530559402771

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