Journal article
Natural phosphorylation of group A streptococcal pyrogenic exotoxin type C
Infection and immunity, Vol.26(2), pp.585-589
11/1979
DOI: 10.1128/IAI.26.2.585-589.1979
PMCID: PMC414656
PMID: 121110
Abstract
Group A streptococcal pyrogenic exotoxin (SPE) type C, produced by strain T18P grown in the presence of 32P, was separated from culture supernatant fluids by using alcohol precipitation. The resulting toxin (EtOH-1) contained 3 X 10(6) to 5 X 10(6) cpm of 32P per milligram of protein. The radiolabel migrated with SPE C during isoelectric focusing in polyacrylamide gels (pI 6.7) and double immunodiffusion, in which the toxin formed a line of identity with highly purified SPE C when reacted with hyperimmune antisera raised against SPE C. The EtOH-1 radiolabeled toxin was pyrogenic and had the capacity to enhance host susceptibility to lethal endotoxin shock. EtOH-1 toxin lost both radiolabel and biological activity after being treated with alkaline phosphatase. The nonspecific lymphocyte mitogenicity of purified unlabeled SPE C was stimulated by adenosine monophosphate but not adenosine, adenosine diphosphate, or adenosine triphosphate. Adenosine monophosphate may function as a cofactor of SPE C and contribute the phosphate group required for biological activity.
Details
- Title: Subtitle
- Natural phosphorylation of group A streptococcal pyrogenic exotoxin type C
- Creators
- P M SchlievertK M BettinD W Watson
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.26(2), pp.585-589
- Publisher
- United States
- DOI
- 10.1128/IAI.26.2.585-589.1979
- PMID
- 121110
- PMCID
- PMC414656
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 11/1979
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001108302771
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