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Naturally occurring antibodies directed to TNBS-modified cell surface but not the trinitrophenyl moiety
Journal article   Peer reviewed

Naturally occurring antibodies directed to TNBS-modified cell surface but not the trinitrophenyl moiety

Zuhair K Ballas and Rudolf C Kuppers
The Journal of immunology (1950), Vol.125(4), pp.1644-1649
10/1980
DOI: 10.4049/jimmunol.125.4.1644
PMID: 7410849

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Abstract

Various normal mouse sera (NMS), as well as murine alloantisera, were found to have antibodies able, in the presence of complement (C), to lyse TNBS-modified, but not unmodified, murine cells. Such lysis was seen irrespective of the H-2 haplotype of the cells and was not effected by either complement or sera alone, but required both. Lytic activity was observed at high serum titers, approximately 1:600 for NMS and up to 1:4000 for alloantisera. It was removed by adsorption with rabbit anti-mouse IgG-Sepharose. The activity could be found in a DEAE-purified IgG fraction, as well as the macroglobulin (IgM) fraction from a Sephadex G-200 column separation of the serum. Several murine anti-H-2 alloantisera were tested for their ability to mediate C dependent lysis of TNBS-modified or unmodified tumor cells. Whereas the alloantisera lysed unmodified tumor cells of the relevant H-2 haplotype only, they lysed cells of different H-2 haplotypes when the latter were modified with TNBS. By various adsorptions of an anti-H-2(k) (B10 α-B10.BR) serum, it was shown that the antibody involved in mediating C lysis of TNBS-modified cells is different from the antibody with anti-H-2(k) specificity. It was shown that this activity was not due to a naturally occurring anti-TNP antibody. The ability of either NMS or anti-H-2(k) to mediate C lysis of TNBS-modified cells was not, in general, decreased by adsorption with TNP-HSA Sepharose. Furthermore, none of the sera tested were able to mediate lysis of tumor cells modified by incubation with TNP-HSA even though the latter were lysed by anti-TNP-KLH. These findings suggest that murine alloantisera and, to a lesser extent, NMS contain antibodies directed to TNBS-modified cell surface proteins but not to the trinitrophenyl (TNP) moiety per se.
 Antibodies Antibody Specificity Immune Sera - pharmacology Rabbits Trinitrobenzenes - pharmacology Mice, Inbred C57BL H-2 Antigens Isoantibodies Immunity, Innate Nitrobenzenes - pharmacology Trinitrobenzenesulfonic Acid - immunology Complement System Proteins Serum Albumin - immunology Animals Mice, Inbred CBA Trinitrobenzenes - immunology Mice Mice, Inbred BALB C Mice, Inbred AKR Trinitrobenzenesulfonic Acid - pharmacology

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