Journal article
Near-Infrared Spectroscopy for Measuring Urea in Hemodialysis Fluids
Clinical chemistry (Baltimore, Md.), Vol.47(7), pp.1279-1286
07/01/2001
DOI: 10.1093/clinchem/47.7.1279
PMID: 11427460
Abstract
AbstractBackground: Near-infrared spectroscopy is proposed as a method for providing real-time urea concentrations during hemodialysis treatments. The feasibility of such noninvasive urea measurements is evaluated in undiluted dialysate fluid.Methods: Near-infrared spectra were collected from calibration solutions of urea prepared in dialysate fluid. Spectra were collected over three distinct spectral regions, and partial least-squares calibration models were optimized and compared for each. Selectivity for urea was demonstrated with two-component samples composed of urea and glucose in the dialysate matrix. The clinical significance of this approach was assessed by measuring urea in real hemodialysate samples.Results: Urea absorptions within the combination and short-wavelength, near-infrared spectral regions provided sufficient spectral information for sound calibration models in the dialysate matrix. The combination spectral region had SEs of calibration (SEC) and prediction (SEP) of 0.38 mmol/L and 0.26 mmol/L, respectively, over the 4720–4600 cm−1 spectral range with 5 partial least-square factors. A second calibration model was established over the combination region from a series of solutions prepared with independently variable concentrations of urea and glucose. The best calibration model for urea in the presence of variable glucose concentrations had a SEC of 0.6 mmol/L and a SEP of 0.4 mmol/L for a 5-factor model over the 4600–4350 cm−1 spectral range. There was no significant decrease in SEP when the 4720–4600 cm−1 calibration model was used to measure urea in real samples collected during actual hemodialysis.Conclusions: Urea can be determined with sufficient sensitivity and selectivity for clinical measurements within the matrix of the hemodialysis fluid.
Details
- Title: Subtitle
- Near-Infrared Spectroscopy for Measuring Urea in Hemodialysis Fluids
- Creators
- Christopher V Eddy - Department of Chemistry and Optical Science and Technology Center, University of Iowa, Iowa City, IA 52242Mark A Arnold - Department of Chemistry and Optical Science and Technology Center, University of Iowa, Iowa City, IA 52242
- Resource Type
- Journal article
- Publication Details
- Clinical chemistry (Baltimore, Md.), Vol.47(7), pp.1279-1286
- DOI
- 10.1093/clinchem/47.7.1279
- PMID
- 11427460
- NLM abbreviation
- Clin Chem
- ISSN
- 0009-9147
- eISSN
- 1530-8561
- Language
- English
- Date published
- 07/01/2001
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216676002771
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