Neonatal Growth Restriction Slows Cardiomyocyte Development and Reduces Adult Heart Size

Madeline H Knott; Sarah E Haskell; Payton E Strawser; Olivia M Rice; Natalie T Bonthius; Vani C Movva; Benjamin E Reinking; Robert D Roghair

doi:10.1002/ar.23851

Back

Neonatal Growth Restriction Slows Cardiomyocyte Development and Reduces Adult Heart Size

Journal article

Open access

Peer reviewed

Neonatal Growth Restriction Slows Cardiomyocyte Development and Reduces Adult Heart Size

Madeline H Knott, Sarah E Haskell, Payton E Strawser, Olivia M Rice, Natalie T Bonthius, Vani C Movva, Benjamin E Reinking and Robert D Roghair

Anatomical record (Hoboken, N.J. : 2007), Vol.301(8), pp.1398-1404

08/2018

DOI: 10.1002/ar.23851

PMCID: PMC6218323

PMID: 29729218

Files and links (1)

url

https://doi.org/10.1002/ar.23851View

Published (Version of record) Open Access

Abstract

Prematurity is associated with reduced cardiac dimensions and an increased risk of cardiovascular disease. While prematurity is typically associated with ex utero neonatal growth restriction (GR), the independent effect of neonatal GR on cardiac development has not been established. We tested the hypothesis that isolated neonatal GR decreases cardiomyocyte growth and proliferation, leading to long-term alterations in cardiac morphology. C57BL/6 mice were fostered in litters ranging in size from 6 to 12 pups to accentuate normal variation in neonatal growth. Regardless of litter size, GR was defined by a weight below the 10th percentile. On postnatal day 8, Ki67 immunoreactivity, cardiomyocyte nucleation status and cardiomyocyte profile area were assessed. For adult mice, cardiomyocyte area was determined, along with cardiac dimensions by echocardiography and cardiac fibrosis by Masson's trichrome stain. On day 8, cardiomyocytes from GR versus control mice were significantly smaller and less likely to be binucleated with evidence of persistent cell cycle activity. As adults, GR mice continued to have smaller cardiomyocytes, as well as decreased left ventricular volumes without signs of fibrosis. Neonatal GR reduces cardiomyocyte size, delays the completion of binucleation, and leads to long-term alterations in cardiac morphology. Clinical studies are needed to ascertain whether these results translate to preterm infants that must continue to grow and mature in the midst of the increased circulatory demands that accompany their premature transition to an ex utero existence. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc.

Age Factors

Animals

Animals, Newborn

Body Weight - physiology

Female

Heart - growth & development

Male

Mice

Mice, Inbred C57BL

Myocytes, Cardiac

Organ Size - physiology

Organogenesis - physiology

Details

Title: Subtitle: Neonatal Growth Restriction Slows Cardiomyocyte Development and Reduces Adult Heart Size
Creators: Madeline H Knott - Roy J. and Lucille A. Carver College of Medicine
Sarah E Haskell - University of Iowa
Payton E Strawser - Roy J. and Lucille A. Carver College of Medicine
Olivia M Rice - University of Iowa
Natalie T Bonthius - Roy J. and Lucille A. Carver College of Medicine
Vani C Movva - Roy J. and Lucille A. Carver College of Medicine
Benjamin E Reinking - University of Iowa
Robert D Roghair - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Anatomical record (Hoboken, N.J. : 2007), Vol.301(8), pp.1398-1404
DOI: 10.1002/ar.23851
PMID: 29729218
PMCID: PMC6218323
NLM abbreviation: Anat Rec (Hoboken)
ISSN: 1932-8486
eISSN: 1932-8494
Grant note: HL102659 / NIH HHS T35 HL007485 / NHLBI NIH HHS R01 HL102659 / NHLBI NIH HHS HL007485 / NIH HHS
Language: English
Date published: 08/2018
Academic Unit: Critical Care; Cardiology; Stead Family Department of Pediatrics; Orthopedics and Rehabilitation; Neonatology
Record Identifier: 9984354120802771

Metrics

8 Record Views

4 Times Cited - Web of Science