Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication with Enhanced Interleukin (IL)-4 and IL-10 Gene Transcripts

SASHA J FACH; DAVID K MEYERHOLZ; JACK M GALLUP; MARK R ACKERMANN; HOWARD D LEHMKUHL; RANDY E SACCO

doi:10.1089/vim.2006.0056

Back

Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication with Enhanced Interleukin (IL)-4 and IL-10 Gene Transcripts

Journal article

Open access

Peer reviewed

Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication with Enhanced Interleukin (IL)-4 and IL-10 Gene Transcripts

SASHA J FACH, DAVID K MEYERHOLZ, JACK M GALLUP, MARK R ACKERMANN, HOWARD D LEHMKUHL and RANDY E SACCO

Viral immunology, Vol.20(1), pp.119-130

2007

DOI: 10.1089/vim.2006.0056

PMCID: PMC2791088

PMID: 17425426

Files and links (1)

url

https://doi.org/10.1089/vim.2006.0056View

Published (Version of record) Open Access

Abstract

The lung microenvironment is constantly exposed to microorganisms and particulate matter. Lung dendritic cells (DCs) play a crucial role in the uptake and processing of antigens found within the respiratory tract. Respiratory syncytial virus (RSV) is a common respiratory tract pathogen in children that induces an influx of DCs to the mucosal surfaces of the lung. Using a neonatal lamb model, we examined the in vivo permissiveness of DCs to RSV infection, as well as overall cell surface changes and cytokine responses of isolated lung DCs after bovine RSV (BRSV) infection. We report that isolated lung DCs and alveolar macrophages support BRSV replication. Isolated lung DCs were determined to be susceptible to BRSV infection as demonstrated by quantification of BRSV non-structural protein 2 mRNA. BRSV infection induced an initial upregulation of CD14 expression on lung DCs, but by 5 d postinfection expression was similar to that on control cells. No significant changes in CD80/86 or MHC class I expression were seen on lung DCs after BRSV infection. Low to moderate expression of MHC class II and DEC-205 was detected by day 5 postinfection. Initially, on day 3 postinfection, lung DCs from BRSV-infected lambs had decreased endocytosis of fluorescein isothiocyanate (FITC)–ovalbumin (OVA). The amount of FITC–OVA endocytosed by lung DCs isolated on day 5 postinfection was similar to that of controls. The most interesting observation was the induction of immunomodulatory interleukin (IL)-4 and IL-10 cytokine gene transcription in lung DCs and alveolar macrophages after in vivo infection with BRSV. Overall, these findings are the first to demonstrate that neonatal lung DCs support in vivo BRSV replication and produce type II cytokines after viral infection.

Details

Title: Subtitle: Neonatal Ovine Pulmonary Dendritic Cells Support Bovine Respiratory Syncytial Virus Replication with Enhanced Interleukin (IL)-4 and IL-10 Gene Transcripts
Creators: SASHA J FACH - Immunobiology Graduate Program, Iowa State University, Ames, Iowa
DAVID K MEYERHOLZ - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
JACK M GALLUP - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
MARK R ACKERMANN - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
HOWARD D LEHMKUHL - Respiratory Diseases Research Unit, National Animal Disease Center, Agricultural Research Services, United States Department of Agriculture, Ames, Iowa
RANDY E SACCO - Immunobiology Graduate Program, Iowa State University, Ames, Iowa
Resource Type: Journal article
Publication Details: Viral immunology, Vol.20(1), pp.119-130
DOI: 10.1089/vim.2006.0056
PMID: 17425426
PMCID: PMC2791088
NLM abbreviation: Viral Immunol
ISSN: 0882-8245
eISSN: 1557-8976
Language: English
Date published: 2007
Academic Unit: Pathology
Record Identifier: 9984083257102771

Metrics

18 Record Views

27 Times Cited - Web of Science