Journal article
Neonatal lipopolysaccharide exposure does not diminish the innate immune response to a subsequent lipopolysaccharide challenge in Holstein bull calves
Journal of dairy science, Vol.99(7), pp.5750-5763
07/01/2016
DOI: 10.3168/jds.2015-10804
PMID: 27108165
Abstract
The innate immune response following experimental mastitis is quite variable between individual dairy cattle. An inflammatory response that minimizes collateral damage to the mammary gland while still effectively resolving the infection following pathogen exposure is beneficial to dairy producers. The ability of a lipopolysaccharide (LPS) exposure in early life to generate a low-responding phenotype and thus reduce the inflammatory response to a later-life LPS challenge was investigated in neonatal hull calves. Ten Holstein bull calves were randomly assigned to either an early life LPS (ELL) group (n = 5) or an early life saline (ELS) group (n = 5). At 7 d of age, calves received either LPS or saline, and at 32 d of age, all calves were challenged with an intravenous dose of LPS to determine the effect of the early life treatment (LPS or saline) on the immune response generated toward a subsequent LPS challenge. Dermal fibroblast and monocyte-derived macrophage cultures from each calf were established at age 20 and 27 d, respectively, to model sustained effects from the early life LPS exposure on gene expression and protein production of components within the ITS response pathway. The ELL calves had greater levels of plasma IL-6 and tumor necrosis factor-a than the ELS calves following the early life LPS or saline treatments. However, levels of these 2 immune markers were similar between ELL and ELS calves when both groups were subsequently challenged with LPS. A comparison of the in vitro LPS responses of the ELL and ELS calves revealed similar patterns of protein production and gene expression following an LPS challenge of both dermal fibroblast and monocyte-derived macrophage cultures established from the treatment groups. Whereas an early life exposure to LPS did not result in a dampened inflammatory response toward a later LPS challenge in these neonatal hull calves, the potential that exposure to inflammation or stress in early life or in utero can create an offspring with a low-responding phenotype as an adult is intriguing and has been documented in rodents. Further work is needed to determine if an inflammatory exposure in utero in a dairy animal would result in a low-responding innate immune phenotype.
Details
- Title: Subtitle
- Neonatal lipopolysaccharide exposure does not diminish the innate immune response to a subsequent lipopolysaccharide challenge in Holstein bull calves
- Creators
- A. L. Benjamin - University of VermontF. T. Korkmaz - University of VermontT. H. Elsasser - Beltsville Agricultural Research CenterD. E. Kerr - University of Vermont
- Resource Type
- Journal article
- Publication Details
- Journal of dairy science, Vol.99(7), pp.5750-5763
- DOI
- 10.3168/jds.2015-10804
- PMID
- 27108165
- NLM abbreviation
- J Dairy Sci
- ISSN
- 0022-0302
- eISSN
- 1525-3198
- Publisher
- Elsevier
- Number of pages
- 14
- Grant note
- VT-HO1417MS / USDA-HATCH grant from the Vermont Agricultural Experiment Station
- Language
- English
- Date published
- 07/01/2016
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696755202771
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