Nervous system function for the testis RNA-binding protein boule in Drosophila

Mei-Ling Joiner; Chun-Fang Wu

doi:10.1080/01677060490477435

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Nervous system function for the testis RNA-binding protein boule in Drosophila

Journal article

Open access

Peer reviewed

Nervous system function for the testis RNA-binding protein boule in Drosophila

Mei-Ling Joiner and Chun-Fang Wu

Journal of neurogenetics, Vol.18(1), pp.341-363

01/2004

DOI: 10.1080/01677060490477435

PMID: 15370196

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Abstract

The RNA-binding protein, Boule is conserved across species and is required for male fertility. Boule and the DAZ homologues in mice and humans appear specific to the testis. Boule functions in spermatogenesis by controlling the translation of the meiotic cell division cycle 25 (Cdc25) phosphatase, Twine. Here we show, for the first time, a function for the DAZ protein, Boule, outside of meiosis. We found that an isoform of Boule is expressed in the nervous system and when its expression is increased we observe mutant phenotypes in neural communication between the receptor and laminar cells of the fly eye, altered larval locomotion and when further overexpressed, viability. As in the germ line, genetic studies indicate that Boule functions in the Cdc25 phosphatase pathway in the nervous system. In a sensitized genetic background of Boule overexpression, we added a loss-of-function mutation of twine and demonstrated a role for Twine Cdc25, in the adult nervous system. Our results indicate that isoforms of boule are expressed outside of the male germ line and that these isoforms have a role in neural function, unlike the boule testis-specific isoform.

Electroretinography

Amino Acid Sequence

RNA-Binding Proteins - genetics

Gene Expression

Animals, Genetically Modified

Molecular Sequence Data

Male

Drosophila Proteins - metabolism

Locomotion

Animals

Interneurons - metabolism

Protein Isoforms - metabolism

Larva

Drosophila - metabolism

Drosophila Proteins - genetics

Photoreceptor Cells, Invertebrate - metabolism

cdc25 Phosphatases - metabolism

RNA-Binding Proteins - metabolism

Protein Isoforms - genetics

Details

Title: Subtitle: Nervous system function for the testis RNA-binding protein boule in Drosophila
Creators: Mei-Ling Joiner - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA. mei-ling-joiner@uiowa.edu
Chun-Fang Wu
Resource Type: Journal article
Publication Details: Journal of neurogenetics, Vol.18(1), pp.341-363
Publisher: England
DOI: 10.1080/01677060490477435
PMID: 15370196
ISSN: 0167-7063
eISSN: 1563-5260
Grant note: NS26528 / NINDS NIH HHS HD18577 / NICHD NIH HHS
Language: English
Date published: 01/2004
Academic Unit: Iowa Neuroscience Institute; Biology; Anesthesia
Record Identifier: 9984070517702771

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