Journal article
Network and Intrinsic Contributions to Carbachol-Induced Oscillations in the Rat Subiculum
Journal of neurophysiology, Vol.86(3), pp.1164-1178
09/01/2001
DOI: 10.1152/jn.2001.86.3.1164
PMID: 11535667
Abstract
Low-frequency network oscillations occur in several areas of the limbic system where they contribute to synaptic plasticity and mnemonic functions that are in turn modulated by cholinergic mechanisms. Here we used slices of the rat subiculum (a limbic area involved in cognitive functions) to establish how network and single neuron (intrinsic) membrane mechanisms participate to the rhythmic oscillations elicited by the cholinergic agent carbachol (CCh, 50–100 μM). We have found that CCh-induced network oscillations (intraoscillatory frequency = 5–16 Hz) are abolished by an antagonist of non- N-methyl-d-aspartate (NMDA) glutamatergic receptors ( n = 6 slices) but persist during blockade of GABA receptors ( n = 16). In addition, during application of glutamate and GABA receptor antagonists, single subicular cells generate burst oscillations at 2.1–6.8 Hz when depolarized with steady current injection. These intrinsic burst oscillations disappear during application of a Ca2+ channel blocker ( n = 6 cells), intracellular Ca2+ chelation ( n = 6), or replacement of extracellular Na+ ( n = 4) but persist in recordings made with electrodes containing a blocker of voltage-gated Na+ channels ( n = 7). These procedures cause similar effects on CCh-induced depolarizing plateau potentials that are contributed by a Ca2+-activated nonselective cationic conductance ( I CAN). Network and intrinsic oscillations along with depolarizing plateau potentials were abolished by the muscarinic receptor antagonist atropine. In conclusion, our findings demonstrate that low-frequency oscillations in the rat subiculum rely on the muscarinic receptor-dependent activation of an intrinsic oscillatory mechanism that is presumably contributed by I CAN and are integrated within the network via non-NMDA receptor-mediated transmission.
Details
- Title: Subtitle
- Network and Intrinsic Contributions to Carbachol-Induced Oscillations in the Rat Subiculum
- Creators
- Margherita D'Antuono - Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec H3A 2B4, Canada;, Istituto di Ricovero e Cura a Carattere Scientifico Neuromed, 86077 Pozzilli (Isernia); andHiroto Kawasaki - Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec H3A 2B4, Canada;, Neurological Institute, Tokyo Women's Medical College, Tokyo 162, JapanCarmela Palmieri - Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec H3A 2B4, Canada;, Ospedale San Paolo, Università degli Studi di Milano, 20142 Milan, ItalyMassimo Avoli - Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec H3A 2B4, Canada;, Istituto di Ricovero e Cura a Carattere Scientifico Neuromed, 86077 Pozzilli (Isernia); and
- Resource Type
- Journal article
- Publication Details
- Journal of neurophysiology, Vol.86(3), pp.1164-1178
- DOI
- 10.1152/jn.2001.86.3.1164
- PMID
- 11535667
- ISSN
- 0022-3077
- eISSN
- 1522-1598
- Language
- English
- Date published
- 09/01/2001
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984083257002771
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