Journal article
NeuCode labels for relative protein quantification
Molecular & cellular proteomics, Vol.13(9), pp.2503-2512
09/2014
DOI: 10.1074/mcp.M114.040287
PMCID: PMC4159665
PMID: 24938287
Abstract
We describe a synthesis strategy for the preparation of lysine isotopologues that differ in mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the proteomes of actively growing cells does not affect cellular proliferation, and we discuss how to use the embedded mass signatures (neutron encoding (NeuCode)) for multiplexed proteome quantification by means of high-resolution mass spectrometry. NeuCode SILAC amalgamates the quantitative accuracy of SILAC with the multiplexing of isobaric tags and, in doing so, offers up new opportunities for biological investigation. We applied NeuCode SILAC to examine the relationship between transcript and protein levels in yeast cells responding to environmental stress. Finally, we monitored the time-resolved responses of five signaling mutants in a single 18-plex experiment.
Details
- Title: Subtitle
- NeuCode labels for relative protein quantification
- Creators
- Anna E Merrill - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706; §Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706Alexander S Hebert - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706Matthew E MacGilvray - ¶Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706Christopher M Rose - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706; §Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706Derek J Bailey - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706Joel C Bradley - ‖Cambridge Isotope Laboratories, Andover, Massachusetts 01810William W Wood - ‖Cambridge Isotope Laboratories, Andover, Massachusetts 01810Marwan El Masri - ‖Cambridge Isotope Laboratories, Andover, Massachusetts 01810Michael S Westphall - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706Audrey P Gasch - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706; ¶Laboratory of Genetics, University of Wisconsin, Madison, Wisconsin 53706Joshua J Coon - From the ‡Genome Center of Wisconsin, University of Wisconsin, Madison, Wisconsin 53706; §Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706; Department of Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706 jcoon@chem.wisc.edu
- Resource Type
- Journal article
- Publication Details
- Molecular & cellular proteomics, Vol.13(9), pp.2503-2512
- DOI
- 10.1074/mcp.M114.040287
- PMID
- 24938287
- PMCID
- PMC4159665
- NLM abbreviation
- Mol Cell Proteomics
- ISSN
- 1535-9476
- eISSN
- 1535-9484
- Publisher
- United States
- Grant note
- R01 GM080148 / NIGMS NIH HHS T32GM008505 / NIGMS NIH HHS 5T32HG002760 / NHGRI NIH HHS R01 GM083989 / NIGMS NIH HHS T32 HG002760 / NHGRI NIH HHS T32 GM008505 / NIGMS NIH HHS
- Language
- English
- Date published
- 09/2014
- Academic Unit
- Pathology; Injury Prevention Research Center
- Record Identifier
- 9984047741602771
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