Neural Pathfinding on Uni- and Multidirectional Photopolymerized Micropatterns

Bradley W Tuft; Linjing Xu; Scott P White; Alison E Seline; Andrew M Erwood; Marlan R Hansen; C. Allan Guymon

doi:10.1021/am501622a

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Neural Pathfinding on Uni- and Multidirectional Photopolymerized Micropatterns

Journal article

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Neural Pathfinding on Uni- and Multidirectional Photopolymerized Micropatterns

Bradley W Tuft, Linjing Xu, Scott P White, Alison E Seline, Andrew M Erwood, Marlan R Hansen and C. Allan Guymon

ACS applied materials & interfaces, Vol.6(14), pp.11265-11276

07/23/2014

DOI: 10.1021/am501622a

PMCID: PMC4215840

PMID: 24911660

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Abstract

Overcoming signal resolution barriers of neural prostheses, such as the commercially available cochlear impant (CI) or the developing retinal implant, will likely require spatial control of regenerative neural elements. To rationally design materials that direct nerve growth, it is first necessary to determine pathfinding behavior of de novo neurite growth from prosthesis-relevant cells such as spiral ganglion neurons (SGNs) in the inner ear. Accordingly, in this work, repeating 90° turns were fabricated as multidirectional micropatterns to determine SGN neurite turning capability and pathfinding. Unidirectional micropatterns and unpatterned substrates are used as comparisons. Spiral ganglion Schwann cell alignment (SGSC) is also examined on each surface type. Micropatterns are fabricated using the spatial reaction control inherent to photopolymerization with photomasks that have either parallel line spacing gratings for unidirectional patterns or repeating 90° angle steps for multidirectional patterns. Feature depth is controlled by modulating UV exposure time by shuttering the light source at given time increments. Substrate topography is characterized by white light interferometry and scanning electron microscopy (SEM). Both pattern types exhibit features that are 25 μm in width and 7.4 ± 0.7 μm in depth. SGN neurites orient randomly on unpatterned photopolymer controls, align and consistently track unidirectional patterns, and are substantially influenced by, but do not consistently track, multidirectional turning cues. Neurite lengths are 20% shorter on multidirectional substrates compared to unidirectional patterns while neurite branching and microfeature crossing events are significantly higher. For both pattern types, the majority of the neurite length is located in depressed surface features. Developing methods to understand neural pathfinding and to guide de novo neurite growth to specific stimulatory elements will enable design of innovative biomaterials that improve functional outcomes of devices that interface with the nervous system.

Details

Title: Subtitle: Neural Pathfinding on Uni- and Multidirectional Photopolymerized Micropatterns
Creators: Bradley W Tuft - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, United States, United States
Linjing Xu - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242, United States, United States
Scott P White - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, United States, United States
Alison E Seline - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242, United States, United States
Andrew M Erwood - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242, United States, United States
Marlan R Hansen - Department of Otolaryngology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242, United States, United States
C. Allan Guymon - Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, Iowa 52242, United States, United States
Resource Type: Journal article
Publication Details: ACS applied materials & interfaces, Vol.6(14), pp.11265-11276
DOI: 10.1021/am501622a
PMID: 24911660
PMCID: PMC4215840
NLM abbreviation: ACS Appl Mater Interfaces
ISSN: 1944-8244
eISSN: 1944-8252
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: R01DC012578; DOI: 10.13039/100000002, name: National Institutes of Health, award: P30DC010362
Language: English
Date published: 07/23/2014
Academic Unit: Dermatology; Molecular Physiology and Biophysics; Pathology; Chemical and Biochemical Engineering; Neurosurgery; Otolaryngology
Record Identifier: 9984006482002771

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