Journal article
Neural Regulation of α‐Dystroglycan Biosynthesis and Glycosylation in Skeletal Muscle
Journal of neurochemistry, Vol.74(1), pp.70-80
01/2000
DOI: 10.1046/j.1471-4159.2000.0740070.x
PMID: 10617107
Abstract
:α‐Dystroglycan (α‐DG) is part of a complex of cell surface proteins linked to dystrophin or utrophin, which is distributed over the myofiber surface and is concentrated at neuromuscular junctions. In laminin overlays of muscle extracts from developing chick hindlimb muscle, α‐DG first appears at embryonic day (E) 10 with an apparent molecular mass of 120 kDa. By E15 it is replaced by smaller (∼100 kDa) and larger (∼150 kDa) isoforms. The larger form increases in amount and in molecular mass (>200 kDa) as the muscle is innervated and the postsynaptic membrane differentiates (E10‐E20), and then decreases dramatically in amount after hatching. In myoblasts differentiating in culture the molecular mass of α‐DG is not significantly increased by their replication, fusion, or differentiation into myotubes. Monoclonal antibody IIH6, which recognizes a carbohydrate epitope on α‐DG, preferentially binds to the larger forms, suggesting that the core protein is differentially glycosylated beginning at E16. Consistent with prior observations implicating the IIH6 epitope in laminin binding, the smaller forms of α‐DG bind more weakly to laminin affinity columns than the larger ones. In blots of adult rat skeletal muscle probed with radiolabeled laminin or monoclonal antibody IIH6, α‐DG appears as a >200‐kDa band that decreases in molecular mass but increases in intensity following denervation. Northern blots reveal a single mRNA transcript, indicating that the reduction in molecular mass of α‐DG after denervation is not obviously a result of alternative splicing but is likely due to posttranslational modification of newly synthesized molecules. The regulation of α‐DG by the nerve and its increased affinity for laminin suggest that glycosylation of this protein may be important in myofiber‐basement membrane interactions during development and after denervation.
Details
- Title: Subtitle
- Neural Regulation of α‐Dystroglycan Biosynthesis and Glycosylation in Skeletal Muscle
- Creators
- Andres LeschzinerHakima MoukhlesMichael LindenbaumStephen H GeeJoanne ButterworthKevin P CampbellSalvatore Carbonetto
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.74(1), pp.70-80
- Publisher
- Blackwell Science Ltd; Oxford UK
- DOI
- 10.1046/j.1471-4159.2000.0740070.x
- PMID
- 10617107
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Number of pages
- 11
- Language
- English
- Date published
- 01/2000
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068382002771
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