Journal article
NeuroD-null mice are deaf due to a severe loss of the inner ear sensory neurons during development
Development (Cambridge), Vol.128(3), pp.417-426
02/2001
DOI: 10.1242/dev.128.3.417
PMCID: PMC2710102
PMID: 11152640
Abstract
A key factor in the genetically programmed development of the nervous system is the death of massive numbers of neurons. Therefore, genetic mechanisms governing cell survival are of fundamental importance to developmental neuroscience. We report that inner ear sensory neurons are dependent on a basic helix-loop-helix transcription factor called NeuroD for survival during differentiation. Mice lacking NeuroD protein exhibit no auditory evoked potentials, reflecting a profound deafness. DiI fiber staining, immunostaining and cell death assays reveal that the deafness is due to the failure of inner ear sensory neuron survival during development. The affected inner ear sensory neurons fail to express neurotrophin receptors, TrkB and TrkC, suggesting that the ability of NeuroD to support neuronal survival may be directly mediated through regulation of responsiveness to the neurotrophins.
Details
- Title: Subtitle
- NeuroD-null mice are deaf due to a severe loss of the inner ear sensory neurons during development
- Creators
- Woo-Young Kim - Department of Molecular Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USABernd Fritzsch - University of Iowa, BiologyAmanda Serls - Department of Molecular Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USALeigh Anne Bakel - Department of Psychology, University of Colorado, Boulder, CO 80309, USAEric J Huang - Pathology Service 113B, VA Medical Center, 4150, San Francisco, CA 94121, USALouis F Reichardt - Program in Neuroscience, Department of Physiology and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USADaniel S Barth - Department of Psychology, University of Colorado, Boulder, CO 80309, USAJacqueline E Lee - Department of Molecular Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
- Resource Type
- Journal article
- Publication Details
- Development (Cambridge), Vol.128(3), pp.417-426
- DOI
- 10.1242/dev.128.3.417
- PMID
- 11152640
- PMCID
- PMC2710102
- NLM abbreviation
- Development
- ISSN
- 0950-1991
- eISSN
- 1477-9129
- Language
- English
- Date published
- 02/2001
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984071676702771
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