Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments

Bernd Fritzsch; Jeremy S Duncan; Jennifer Kersigo; Brian Gray; Karen L Elliott

doi:10.1007/978-1-4939-3615-1_14

Back

Journal article

Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments

Bernd Fritzsch, Jeremy S Duncan, Jennifer Kersigo, Brian Gray and Karen L Elliott

Methods in molecular biology (Clifton, N.J.), Vol.1427, pp.243-262

2016

DOI: 10.1007/978-1-4939-3615-1_14

PMCID: PMC4993453

PMID: 27259931

Files and links (1)

url

http://doi.org/10.1007/978-1-4939-3615-1_14View

Open Access

Abstract

The inner ear has long been at the cutting edge of tract tracing techniques that have shaped and reshaped our understanding of the ear's innervation patterns. This review provides a historical framework to understand the importance of these techniques for ear innervation and for development of tracing techniques in general; it is hoped that lessons learned will help to quickly adopt transformative novel techniques and their information and correct past beliefs based on technical limitations. The technical part of the review presents details of our protocol as developed over the last 30 years. We also include arguments as to why these recommendations work best to generate the desired outcome of distinct fiber and cell labeling, and generate reliable data for any investigation. We specifically focus on two tracing techniques, in part developed and/or championed for ear innervation analysis: the low molecular multicolor dextran amine tract tracing technique and the multicolor tract tracing technique with lipophilic dyes.

Ear - anatomy & histology

Fluorescent Dyes - chemistry

Animals

Neuroanatomical Tract-Tracing Techniques - methods

Dextrans - chemistry

Mice

Ear - innervation

Details

Title: Subtitle: Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments
Creators: Bernd Fritzsch - Department of Biology, CLAS, University of Iowa, 356 Medical Research Center, Iowa City, IA, 52242, USA. bernd-fritzsch@uiowa.edu
Jeremy S Duncan - Division of Otolaryngology, University of Utah, Salt Lake City, UT, USA
Jennifer Kersigo - Department of Biology, CLAS, University of Iowa, 356 Medical Research Center, Iowa City, IA, 52242, USA
Brian Gray - Molecular Targeting Technologies, Inc., West Chester, PA, USA
Karen L Elliott - Department of Biology, CLAS, University of Iowa, 356 Medical Research Center, Iowa City, IA, 52242, USA
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.1427, pp.243-262
DOI: 10.1007/978-1-4939-3615-1_14
PMID: 27259931
PMCID: PMC4993453
NLM abbreviation: Methods Mol Biol
eISBN: 9781493936151; 1493936158
ISSN: 1064-3745
eISSN: 1940-6029
Publisher: United States
Grant note: R01 DC005590 / NIDCD NIH HHS R43 GM108470 / NIGMS NIH HHS P30 DC010362 / NIDCD NIH HHS
Language: English
Date published: 2016
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070663602771

Metrics

23 Record Views

15 Times Cited - Web of Science