Journal article
Neuroblastoma cells inhibit the immunostimulatory function of dendritic cells
Journal of pediatric surgery, Vol.38(6), pp.901-905
2003
DOI: 10.1016/S0022-3468(03)00119-2
PMID: 12778389
Abstract
Dendritic cells (DC) are critical for induction of antitumor immunity. Recent studies suggest that tumors may avoid immune destruction by inhibiting DC function. The authors investigated the effect of neuroblastoma (NB) on surface antigen expression and T cell activation by DCs.
DCs were generated in the presence of granulocyte and macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) from peripheral blood of healthy donors. On day 6 of culture, DCs were exposed to human NB cells and were analyzed by flow cytometry.
The proinflammatory cytokine tumor necrosis factor alpha (TNF-α) failed to upregulate the expression of HLA-DR and costimulatory molecule CD86 by DCs that were cultured with NB. Conversely, upregulation was preserved when DCs were cultured in the absence of NB. Exposure to NB also led to apoptosis of DCs as shown by 2-fold increase in surface phosphatidylserine. It appears that direct contact was required to inhibit DC maturation, because DCs separated from NB cells using a transwell insert did not suppress surface antigen expression. Finally, DCs exposed to NB inhibited the proliferation of allogeneic T cells in mixed lymphocyte reactions.
These findings have significant implications for tumor-pulsed DC vaccines in the treatment of NB and suggest a mechanism by which NB escape rejection.
Details
- Title: Subtitle
- Neuroblastoma cells inhibit the immunostimulatory function of dendritic cells
- Creators
- Xiao Chen - Children’s Hospital of Wisconsin, Milwaukee, WI, USAKara Doffek - Children’s Hospital of Wisconsin, Milwaukee, WI, USASonia L Sugg - Children’s Hospital of Wisconsin, Milwaukee, WI, USAJoel Shilyansky - Children’s Hospital of Wisconsin, Milwaukee, WI, USA
- Resource Type
- Journal article
- Publication Details
- Journal of pediatric surgery, Vol.38(6), pp.901-905
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0022-3468(03)00119-2
- PMID
- 12778389
- ISSN
- 0022-3468
- eISSN
- 1531-5037
- Language
- English
- Date published
- 2003
- Academic Unit
- Stead Family Department of Pediatrics; Surgery
- Record Identifier
- 9984051545502771
Metrics
11 Record Views