Journal article
Neurochemical changes in the developing rat hippocampus during prolonged hypoglycemia
Journal of neurochemistry, Vol.114(3), pp.728-738
08/01/2010
DOI: 10.1111/j.1471-4159.2010.06797.x
PMCID: PMC2910187
PMID: 20477939
Abstract
P>Hypoglycemia is common during development and is associated with the risk of neurodevelopmental deficits in human infants. The effects of hypoglycemia on the developing hippocampus are poorly understood. The sequential changes in energy substrates, amino acids and phosphocreatine were measured from the hippocampus during 180 min of insulin-induced hypoglycemia (blood glucose < 2.5 mmol/L) in 14-day-old rats using in vivo1H NMR spectroscopy. Hypoglycemia resulted in neuroglycopenia (brain glucose < 0.5 mu mol/g). However, the phosphocreatine/creatine (PCr/Cr) ratio was maintained in the physiological range until approximately 150 min of hypoglycemia, indicating that energy supply was sufficient to meet the energy demands. Lactate concentration decreased soon after the onset of neuroglycopenia. Beyond 60 min, glutamine and glutamate became the major energy substrates. A precipitous decrease in the PCr/Cr ratio, indicative of impending energy failure occurred only after significant depletion of these amino acids. Once glutamate and glutamine were significantly exhausted, aspartate became the final energy source. N-acetylaspartate concentration remained unaltered, suggesting preservation of neuronal/mitochondrial integrity during hypoglycemia. Correction of hypoglycemia normalized the PCr/Cr ratio and partially restored the amino acids to pre-hypoglycemia levels. Compensatory neurochemical changes maintain energy homeostasis during prolonged hypoglycemia in the developing hippocampus.
Details
- Title: Subtitle
- Neurochemical changes in the developing rat hippocampus during prolonged hypoglycemia
- Creators
- Raghavendra Rao - University of MinnesotaKathleen Ennis - University of MinnesotaJeffery D. Long - University of MinnesotaKamil Ugurbil - University of MinnesotaRolf Gruetter - University of MinnesotaIvan Tkac - University of Minnesota
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.114(3), pp.728-738
- Publisher
- Wiley
- DOI
- 10.1111/j.1471-4159.2010.06797.x
- PMID
- 20477939
- PMCID
- PMC2910187
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Number of pages
- 11
- Grant note
- University Pediatrics Foundation K08HD047276 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) W. M. Keck Foundation; W.M. Keck Foundation P30NS057091 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) Graduate School, University of Minnesota; University of Minnesota System K08 HD47276; P41 RR008079; P30 NS057091 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P41RR008079 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) Viking Children's Fund
- Language
- English
- Date published
- 08/01/2010
- Academic Unit
- Psychiatry; Biostatistics
- Record Identifier
- 9984280839702771
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