Journal article
Neuroglobin and cytoglobin distribution in the anterior eye segment: a comparative immunohistochemical study
The journal of histochemistry and cytochemistry, Vol.56(9), pp.863-872
09/2008
DOI: 10.1369/jhc.2008.951392
PMCID: PMC2516955
PMID: 18574250
Abstract
This study provides a detailed description of immunolocalization of two oxygen-binding proteins, neuroglobin (Ngb) and cytoglobin (Cygb), in the anterior segment of healthy human and canine eyes. Specific antibodies against Ngb and Cygb were used to examine their distribution patterns in anterior segment structures including the cornea, iris, trabecular meshwork, canal of Schlemm, ciliary body, and lens. Patterns of immunoreactivity (IR) were imaged with confocal scanning laser and conventional microscopy. Analysis of sectioned human and canine eyes showed Ngb and Cygb IR in the corneal epithelium and endothelium. In the iris, Ngb and Cygb IR was localized to the anterior border and the stroma, iridal sphincter, and dilator muscle. In the iridocorneal angle, Ngb and Cygb were detected in endothelial cells of the trabecular meshwork and canal of Schlemm in human. In the ciliary body, Ngb and Cygb IR was localized to the non-pigmented ciliary epithelium of the pars plana and pars plicata and in ciliary body musculature. Ngb and Cygb distribution was similar and colocalized within the same structures of healthy human and canine anterior eye segments. Based on their immunolocalization and previously reported biochemical features, we hypothesize that Ngb and Cygb may function as scavengers of reactive oxygen species. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
Details
- Title: Subtitle
- Neuroglobin and cytoglobin distribution in the anterior eye segment: a comparative immunohistochemical study
- Creators
- Jelena Ostojic - Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USASinisa GrozdanicNasreen A SyedMark S HargroveJames T Trent IIIMarkus H KuehnRandy H KardonYoung H KwonDonald S Sakaguchi
- Resource Type
- Journal article
- Publication Details
- The journal of histochemistry and cytochemistry, Vol.56(9), pp.863-872
- DOI
- 10.1369/jhc.2008.951392
- PMID
- 18574250
- PMCID
- PMC2516955
- NLM abbreviation
- J Histochem Cytochem
- ISSN
- 0022-1554
- eISSN
- 1551-5044
- Publisher
- United States
- Grant note
- R01 EY019485 / NEI NIH HHS 44007 / PHS HHS
- Language
- English
- Date published
- 09/2008
- Academic Unit
- Pathology; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980040202771
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