Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons

Jelena Ostojić; Donald S Sakaguchi; Yancy de Lathouder; Mark S Hargrove; James T Trent III; Young H Kwon; Randy H Kardon; Markus H Kuehn; Daniel M Betts; Sinisa Grozdanić

doi:10.1167/iovs.05-0465

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Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons

Journal article

Peer reviewed

Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons

Jelena Ostojić, Donald S Sakaguchi, Yancy de Lathouder, Mark S Hargrove, James T Trent III, Young H Kwon, Randy H Kardon, Markus H Kuehn, Daniel M Betts and Sinisa Grozdanić

Investigative ophthalmology & visual science, Vol.47(3), pp.1016-1023

03/2006

DOI: 10.1167/iovs.05-0465

PMID: 16505036

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Abstract

The goal of this study was to describe the detailed localization of the novel oxygen-binding molecules, neuroglobin (Ngb) and cytoglobin (Cygb), in mammalian retinas and to determine whether Ngb and Cygb are neuronal or glial proteins in the retina. Antibodies directed against Ngb and Cygb were used to examine their patterns of distribution in normal canine retinas. Immunoblot analysis was performed to verify antibody specificity and the presence of Ngb and Cygb in canine tissues. Double-labeling immunohistochemistry was performed with the Ngb and Cygb antibodies along with antibodies against neuronal (MAP-2, class III beta-tubulin (TUJ1), PKCalpha, and calretinin) and glial antigens (vimentin and CRALBP). Tissue sections were analyzed with light and confocal microscopy. Ngb and Cygb proteins were observed in different retinal cells. Cygb (but not Ngb) was also present in canine kidney, liver, lung, and heart tissue. Immunohistochemical analysis of canine retinas demonstrated Ngb immunoreactivity (IR) in the ganglion cell layer (GCL), inner (INL) and outer (ONL) nuclear layers, inner (IPL) and outer plexiform (OPL) layers, photoreceptor inner segments (IS), and retinal pigment epithelium (RPE). Ngb IR was localized within retinal neurons, but not in glia. Cygb IR was found in neurons and their processes in the GCL, IPL, INL, and OPL and within the RPE, but not in glia. Ngb and Cygb are widely distributed in retinal neurons and RPE, but not in glial cells of the canine retina. Their structure and distribution is suggestive of a possible role in oxygen transport in the mammalian retina.

Retina - metabolism

Vimentin - metabolism

Nuclear Proteins - metabolism

Globins - metabolism

Retinaldehyde - metabolism

Blotting, Western

Oxygen - metabolism

Nerve Tissue Proteins - metabolism

S100 Calcium Binding Protein G - metabolism

Microscopy, Confocal

Tubulin - metabolism

Animals

Calbindin 2

Dogs

Neuroglia - metabolism

Neurons - metabolism

Protein Kinase C-alpha

Fluorescent Antibody Technique, Indirect

Details

Title: Subtitle: Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons
Creators: Jelena Ostojić - Department of Genetics, Development and Cell Biology and Interdepartmental Neuroscience Program, Iowa State University, Ames 50011, USA
Donald S Sakaguchi
Yancy de Lathouder
Mark S Hargrove
James T Trent III
Young H Kwon
Randy H Kardon
Markus H Kuehn
Daniel M Betts
Sinisa Grozdanić
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.47(3), pp.1016-1023
DOI: 10.1167/iovs.05-0465
PMID: 16505036
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Language: English
Date published: 03/2006
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983980392502771

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