Neuroinflammation in advanced canine glaucoma

Bing Jiang; Matthew M Harper; Helga Kecova; Grazyna Adamus; Randy H Kardon; Sinisa D Grozdanic; Markus H Kuehn

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Neuroinflammation in advanced canine glaucoma

Journal article

Peer reviewed

Neuroinflammation in advanced canine glaucoma

Bing Jiang, Matthew M Harper, Helga Kecova, Grazyna Adamus, Randy H Kardon, Sinisa D Grozdanic and Markus H Kuehn

Molecular vision, Vol.16(225-26), pp.2092-2108

10/15/2010

PMCID: PMC2965571

PMID: 21042562

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Abstract

The pathophysiological events that occur in advanced glaucoma are not well characterized. The principal purpose of this study is to characterize the gene expression changes that occur in advanced glaucoma. Retinal RNA was obtained from canine eyes with advanced glaucoma as well as from healthy eyes. Global gene expression patterns were determined using oligonucleotide microarrays and confirmed by real-time PCR. The presence of tumor necrosis factor (TNF) and its receptors was evaluated by immunolabeling. Finally, we evaluated the presence of serum autoantibodies directed against retinal epitopes using western blot analyses. We identified over 500 genes with statistically significant changes in expression level in the glaucomatous retina. Decreased expression levels were detected for large number of functional groups, including synapse and synaptic transmission, cell adhesion, and calcium metabolism. Many of the molecules with decreased expression levels have been previously shown to be components of retinal ganglion cells. Genes with elevated expression in glaucoma are largely associated with inflammation, such as antigen presentation, protein degradation, and innate immunity. In contrast, expression of many other pro-inflammatory genes, such as interferons or interleukins, was not detected at abnormal levels. This study characterizes the molecular events that occur in the canine retina with advanced glaucoma. Our data suggest that in the dog this stage of the disease is accompanied by pronounced retinal neuroinflammation.

Immunohistochemistry

Inflammation - pathology

Reproducibility of Results

Autoantibodies - blood

Gene Expression Regulation

Gene Expression Profiling

Glaucoma - complications

Reverse Transcriptase Polymerase Chain Reaction

Glaucoma - blood

Inflammation - complications

Animals

Eye Proteins - metabolism

Nervous System - pathology

Dogs

Inflammation - genetics

Antigens - immunology

Glaucoma - pathology

Eye Proteins - genetics

Glaucoma - genetics

Retina - pathology

Details

Title: Subtitle: Neuroinflammation in advanced canine glaucoma
Creators: Bing Jiang - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA 52242, USA
Matthew M Harper
Helga Kecova
Grazyna Adamus
Randy H Kardon
Sinisa D Grozdanic
Markus H Kuehn
Resource Type: Journal article
Publication Details: Molecular vision, Vol.16(225-26), pp.2092-2108
PMID: 21042562
PMCID: PMC2965571
NLM abbreviation: Mol Vis
ISSN: 1090-0535
eISSN: 1090-0535
Publisher: United States
Grant note: R01 EY019485 / NEI NIH HHS EY019485 / NEI NIH HHS EY1305 / NEI NIH HHS
Language: English
Date published: 10/15/2010
Academic Unit: Iowa Neuroscience Institute; Biology; Ophthalmology and Visual Sciences
Record Identifier: 9983980042802771

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