Journal article
Neuronal Differentiation of Induced Pluripotent Stem Cells on Surfactant Templated Chitosan Hydrogels
Biomacromolecules, Vol.17(5), pp.1684-1695
05/09/2016
DOI: 10.1021/acs.biomac.6b00098
PMID: 27008004
Abstract
The development of effective tissue engineering materials requires careful consideration of several properties beyond biocompatibility, including permeability and mechanical stiffness. While surfactant templating has been used for over a decade to control the physical properties of photopolymer materials, the potential benefit of this technique with regard to biomaterials has yet to be fully explored. Herein we demonstrate that surfactant templating can be used to tune the water uptake and compressive modulus of photo-cross-linked chitosan hydrogels. Interestingly, templating with quaternary ammonium surfactants also hedges against property fluctuations that occur with changing pH. Further, we demonstrate that, after adequate surfactant removal, these materials are nontoxic, support the attachment of induced pluripotent stem cells and facilitate stem cell differentiation to neuronal phenotypes. These results demonstrate the utility of surfactant templating for optimizing the properties of biomaterials intended for a variety of applications, including retinal regeneration.
Details
- Title: Subtitle
- Neuronal Differentiation of Induced Pluripotent Stem Cells on Surfactant Templated Chitosan Hydrogels
- Creators
- Kristan S Worthington - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United StatesBrian J Green - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United StatesMary Rethwisch - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United StatesLuke A Wiley - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United StatesBudd A Tucker - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United StatesC Allan Guymon - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United StatesAliasger K Salem - Department of Chemical and Biochemical Engineering, ‡Wynn Institute for Vision Research, Department of Ophthalmology and Visual Sciences, and §Division of Pharmaceutics and Translational Therapeutics, Department of Pharmaceutical Sciences and Experimental Therapeutics, The University of Iowa , Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Biomacromolecules, Vol.17(5), pp.1684-1695
- DOI
- 10.1021/acs.biomac.6b00098
- PMID
- 27008004
- NLM abbreviation
- Biomacromolecules
- ISSN
- 1525-7797
- eISSN
- 1526-4602
- Publisher
- United States
- Grant note
- DOI: 10.13039/100000052, name: NIH Office of the Director, award: 1 DP2 OD007483-01; DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: 1 R21 DEO2420601A1; DOI: 10.13039/100000146, name: Division of Chemical, Bioengineering, Environmental, and Transport Systems, award: 0933450, 1438486; DOI: 10.13039/100000002, name: National Institutes of Health, award: 1 R01 024605-01; name: Stephen A. Wynn Institute for Vision Research, University of Iowa
- Language
- English
- Date published
- 05/09/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980092502771
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