Journal article
Neuronal Expression of chimeric genes in transgenic mice
Neuron (Cambridge, Mass.), Vol.1(4), pp.311-320
1988
DOI: 10.1016/0896-6273(88)90079-7
PMID: 3078520
Abstract
Gene expression may occur in unexpected ectopic sites when diverse genetic elements are juxtaposed as chimeric genes in transgenic mice. To determine the specific contribution of the promoter and reporter gene in ectopic expression, we have analyzed the expression of 14 different fusion genes in transgenic mice. Chimeric genes containing the mouse metallothionein-I promoter linked to either the rat or human growth hormone gene or the calcitonin/CGRP gene are expressed in a very similar pattern of neuronal regions. This ectopic expression is not a unique feature of the metallothionein promoter, since transferring the human growth hormone gene to four other heterologous promoters resulted in varying degrees of ectopic expression in overlapping subsets of cortical and hypothalamic neurons. The novel pattern of ectopic expression suggests that these otherwise unrelated neurons share a common developmental regulatory machinery for activation of gene transcription.
Details
- Title: Subtitle
- Neuronal Expression of chimeric genes in transgenic mice
- Creators
- Andrew F Russo - Center for Molecular Genetics and Eukaryotic Regulatory Biology Program, M-013, USAE Bryan Crenshaw - Center for Molecular Genetics and Eukaryotic Regulatory Biology Program, M-013, USASergio A Lira - Center for Molecular Genetics and Eukaryotic Regulatory Biology Program, M-013, USADonna M Simmons - Howard Hughes Medical Institute, USALarry W Swanson - Howard Hughes Medical Institute, USAMichael G Rosenfeld - Center for Molecular Genetics and Eukaryotic Regulatory Biology Program, M-013, USA
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.1(4), pp.311-320
- DOI
- 10.1016/0896-6273(88)90079-7
- PMID
- 3078520
- NLM abbreviation
- Neuron
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1988
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center
- Record Identifier
- 9984020864102771
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