Neuronal Mechanisms of Learning and Memory Revealed by Spatial and Temporal Suppression of Neurotransmission Using Shibirets1, a Temperature-Sensitive Dynamin Mutant Gene in Drosophila Melanogaster

Junko Kasuya; Hiroshi Ishimoto; Toshihiro Kitamoto

doi:10.3389/neuro.02.011.2009

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Neuronal Mechanisms of Learning and Memory Revealed by Spatial and Temporal Suppression of Neurotransmission Using Shibirets1, a Temperature-Sensitive Dynamin Mutant Gene in Drosophila Melanogaster

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Neuronal Mechanisms of Learning and Memory Revealed by Spatial and Temporal Suppression of Neurotransmission Using Shibirets1, a Temperature-Sensitive Dynamin Mutant Gene in Drosophila Melanogaster

Junko Kasuya, Hiroshi Ishimoto and Toshihiro Kitamoto

Frontiers in molecular neuroscience, Vol.2, 11

08/20/2009

DOI: 10.3389/neuro.02.011.2009

PMCID: PMC2737436

PMID: 19738923

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Abstract

The fruit fly Drosophila melanogaster is an excellent model organism to identify genes and genetic pathways important for learning and memory. However, its small size makes surgical treatment and electrophysiological manipulation technically difficult, hampering the functional analysis of neuronal circuits that play critical roles in memory processing. To circumvent this problem, we developed a unique experimental strategy that uses the temperature-sensitive allele of the Drosophila dynamin gene, shibire ts1 ( shi ts1 ), in combination with the GAL4/UAS expression system. This strategy allows for rapid and reversible perturbation of synaptic neurotransmission in identifiable neurons, and analysis of the behavioral consequences of such manipulation in free-moving animals. Since its introduction in 2001, this GAL4/UAS- shi ts1 strategy has been widely used to study the neuronal basis of learning and memory. This review focuses on how this strategy has revitalized Drosophila memory research, and contributed to our understanding of dynamic neuronal processes that control various aspects of memory.

Neuroscience

learning and memory

temperature-sensitive mutant

Drosophila

dynamin

Details

Title: Subtitle: Neuronal Mechanisms of Learning and Memory Revealed by Spatial and Temporal Suppression of Neurotransmission Using Shibirets1, a Temperature-Sensitive Dynamin Mutant Gene in Drosophila Melanogaster
Creators: Junko Kasuya - Department of Anesthesia, Carver College of Medicine, University of Iowa
Hiroshi Ishimoto - Department of Anesthesia, Carver College of Medicine, University of Iowa
Toshihiro Kitamoto - Department of Anesthesia, Carver College of Medicine, University of Iowa
Resource Type: Journal article
Publication Details: Frontiers in molecular neuroscience, Vol.2, 11
DOI: 10.3389/neuro.02.011.2009
PMID: 19738923
PMCID: PMC2737436
NLM abbreviation: Front Mol Neurosci
ISSN: 1662-5099
eISSN: 1662-5099
Publisher: Frontiers Research Foundation
Language: English
Date published: 08/20/2009
Academic Unit: Iowa Neuroscience Institute; Anesthesia; Neuroscience and Pharmacology
Record Identifier: 9984006483402771

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