Journal article
Neuronal expression and regulation of CGRP promoter activity following viral gene transfer into cultured trigeminal ganglia neurons
Brain research, Vol.997(1), pp.103-110
2004
DOI: 10.1016/j.brainres.2003.11.005
PMID: 14715155
Abstract
We have examined the regulation of calcitonin gene-related peptide (CGRP) promoter activity in primary cultures of rat trigeminal ganglia neurons. A viral vector was used to circumvent the potential complication of examining only a small subpopulation of cells in the heterogeneous cultures. Infection with high titers of recombinant adenovirus containing 1.25 kb of the rat CGRP promoter linked to the β-galactosidase reporter gene (AdCGRP–
lacZ) yielded expression in about 50% of the CGRP-expressing neurons. The CGRP–
lacZ reporter gene was preferentially expressed in neurons, with 91% co-expression with endogenous CGRP. In contrast, an adenoviral vector containing a CMV–
lacZ reporter was predominantly expressed in non-neuronal cells, with only 29% co-expression with CGRP. We then asked whether the CGRP promoter in the viral vector could be regulated by serotonin receptor type 1 (5-HT
1) agonists. Promoter activity was decreased two- to threefold by treatment with five 5-HT
1B/D agonists, including the triptan drugs sumatriptan, eletriptan, and rizatriptan that are used for migraine treatment. As controls, CMV promoter activity was not affected, and 5-HT
1B/D receptor antagonists blocked the repression caused by sumatriptan and eletriptan. Thus, adenoviral gene transfer can be used in trigeminal ganglia neurons for studying the mechanisms of triptan drug action on CGRP synthesis.
Details
- Title: Subtitle
- Neuronal expression and regulation of CGRP promoter activity following viral gene transfer into cultured trigeminal ganglia neurons
- Creators
- Paul L Durham - Department of Biology, Southwest Missouri State University, Springfield, MO 65804, USAPenny X Dong - Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USAKevin T Belasco - Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USAJeffrey Kasperski - Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USAWilliam W Gierasch - Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USALars Edvinsson - Department of Internal Medicine, Lund University Hospital, S-221 85, Lund, SwedenDonald D Heistad - Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USAFrank M Faraci - Department of Pharmacology, University of Iowa, Iowa City, IA 52242, USAAndrew F Russo - Department of Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Brain research, Vol.997(1), pp.103-110
- DOI
- 10.1016/j.brainres.2003.11.005
- PMID
- 14715155
- NLM abbreviation
- Brain Res
- ISSN
- 0006-8993
- eISSN
- 1872-6240
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2004
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Cardiovascular Medicine; Craniofacial Anomalies Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984020851802771
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