Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions

Dake Huang; Richie Zhang; Silvia Gasparini; Miriam C McDonough; William J Paradee; Joel C Geerling

doi:10.1002/cne.25400

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Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions

Journal article

Open access

Peer reviewed

Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions

Dake Huang, Richie Zhang, Silvia Gasparini, Miriam C McDonough, William J Paradee and Joel C Geerling

Journal of comparative neurology (1911), Vol.530(18), pp.3157-3178

08/29/2022

DOI: 10.1002/cne.25400

PMCID: PMC9588594

PMID: 36036349

Appears in UI Libraries Support Open Access

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Abstract

Neuropeptide S (NPS) increases wakefulness. A small number of neurons in the brainstem express Nps. These neurons are located in or near the parabrachial nucleus (PB), but we know very little about their ontogeny, connectivity, and function. To identify Nps-expressing neurons within the molecular framework of the PB region, we used in situ hybridization, immunofluorescence, and Cre-reporter labeling in mice. The primary concentration of Nps-expressing neurons borders the lateral lemniscus at far-rostral levels of the lateral PB. Caudal to this main cluster, Nps-expressing neurons scatter through the PB and form a secondary concentration medial to the locus coeruleus (LC). Most Nps-expressing neurons in the PB region are Atoh1-derived, Foxp2-expressing, and mutually exclusive with neurons expressing Calca or Lmx1b. Among Foxp2-expressing PB neurons, those expressing Nps are distinct from intermingled subsets expressing Cck or Pdyn. Examining Nps Cre-reporter expression throughout the brain identified novel populations of neurons in the nucleus incertus, anterior hypothalamus, and lateral habenula. This information will help focus experimental questions about the connectivity and function of NPS neurons.

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Details

Title: Subtitle: Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions
Creators: Dake Huang - University of Iowa
Richie Zhang - University of Iowa
Silvia Gasparini - University of Iowa
Miriam C McDonough - University of Iowa
William J Paradee - University of Iowa
Joel C Geerling - University of Iowa
Resource Type: Journal article
Publication Details: Journal of comparative neurology (1911), Vol.530(18), pp.3157-3178
DOI: 10.1002/cne.25400
PMID: 36036349
PMCID: PMC9588594
NLM abbreviation: J Comp Neurol
eISSN: 1096-9861
Publisher: Wiley
Grant note: DOI: 10.13039/100000065, name: National Institute of Neurological Disorders and Stroke, award: NS099425
Language: English
Date published: 08/29/2022
Academic Unit: Neurology; Iowa Neuroscience Institute; Medicine Administration
Record Identifier: 9984297557602771

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