Journal article
Neuroprotective effects and magnetic resonance imaging of mesenchymal stem cells labeled with SPION in a rat model of Huntington's disease
Stem cell research, Vol.9(2), pp.143-155
09/01/2012
DOI: 10.1016/j.scr.2012.05.005
PMID: 22742973
Abstract
Bone marrow mesenchymal stem cells (MSC) have been tested and proven effective in some neurodegenerative diseases, but their tracking after transplantation may be challenging. Our group has previously demonstrated the feasibility and biosafety of rat MSC labeling with iron oxide superparamagnetic nanoparticles (SPION). In this study, we investigated the therapeutic potential of SPION-labeled MSC in a rat model of Huntington's disease, a genetic degenerative disease with characteristic deletion of striatal GABAergic neurons. MSC labeled with SPION were injected into the striatum 1 h after quinolinic acid injection. FJ-C analysis demonstrated that MSC transplantation significantly decreased the number of degenerating neurons in the damaged striatum 7 days after lesion. In this period, MSC transplantation enhanced the striatal expression of FGF-2 but did not affect subventricular zone proliferation, as demonstrated by Ki67 proliferation assay. In addition, MSC transplantation significantly reduced the ventriculomegaly in the lesioned brain. MRI and histological techniques detected the presence of the SPION-labeled cells at the lesion site. SPION-labeled MSC produced magnetic resonance imaging (MRI) signals that were visible for at least 60 days after transplantation. Our data highlight the potential of adult MSC to reduce brain damage under neurodegenerative diseases and indicate the use of nanoparticles in cell tracking, supporting their potential as valuable tools for cell therapy. (C) 2012 Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Neuroprotective effects and magnetic resonance imaging of mesenchymal stem cells labeled with SPION in a rat model of Huntington's disease
- Creators
- Louise Moraes - Universidade Federal do Rio de JaneiroAndreia Vasconcelos-dos-Santos - Universidade Federal do Rio de JaneiroFernando Cleber Santana - Universidade Federal do Rio de JaneiroMariana Araya Godoy - Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Ctr Ciencias Saude, BR-21941590 Rio De Janeiro, BrazilPaulo Henrique Rosado-de-Castro - Universidade Federal do Rio de JaneiroJasmin - Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Ctr Ciencias Saude, BR-21941590 Rio De Janeiro, BrazilRicardo Luiz Azevedo-Pereira - Universidade Federal do Rio de JaneiroWagner Monteiro Cintra - Universidade Federal do Rio de JaneiroEmerson Leandro Gasparetto - Universidade Federal do Rio de JaneiroMarcelo Felippe Santiago - Universidade Federal do Rio de JaneiroRosalia Mendez-Otero - Universidade Federal do Rio de Janeiro
- Resource Type
- Journal article
- Publication Details
- Stem cell research, Vol.9(2), pp.143-155
- DOI
- 10.1016/j.scr.2012.05.005
- PMID
- 22742973
- NLM abbreviation
- Stem Cell Res
- ISSN
- 1873-5061
- eISSN
- 1876-7753
- Publisher
- Elsevier
- Number of pages
- 13
- Grant note
- Instituto Nacional de Ciencia e Tecnologia de Biologia Estrutural e Bioimagem (Inbeb) Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) Ministry of Health (MS/SCTIE/DECIT) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ); Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio De Janeiro (FAPERJ)
- Language
- English
- Date published
- 09/01/2012
- Academic Unit
- Radiology
- Record Identifier
- 9985143125402771
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