Neutralization of Multiple Staphylococcal Superantigens by a Single-Chain Protein Consisting of Affinity-Matured, Variable Domain Repeats

Xi Yang; Rebecca A Buonpane; Beenu Moza; A. K. M. Nur-ur Rahman; Ningyan Wang; Patrick M Schlievert; John K McCormick; Eric J Sundberg; David M Kranz

doi:10.1086/589776

Back

Neutralization of Multiple Staphylococcal Superantigens by a Single-Chain Protein Consisting of Affinity-Matured, Variable Domain Repeats

Journal article

Open access

Peer reviewed

Neutralization of Multiple Staphylococcal Superantigens by a Single-Chain Protein Consisting of Affinity-Matured, Variable Domain Repeats

Xi Yang, Rebecca A Buonpane, Beenu Moza, A. K. M. Nur-ur Rahman, Ningyan Wang, Patrick M Schlievert, John K McCormick, Eric J Sundberg and David M Kranz

The Journal of infectious diseases, Vol.198(3), pp.344-348

08/01/2008

DOI: 10.1086/589776

PMCID: PMC2649774

PMID: 18522504

Files and links (1)

url

https://doi.org/10.1086/589776View

Published (Version of record) Open Access

Abstract

Staphylococcus aureus secretes various toxins that act as superantigens by stimulating a large fraction of the host's T cells. Toxin binding to variable domains of T cell receptor β chains (Vβ) leads to massive release of inflammatory molecules and potentially to toxic shock syndrome (TSS). Previously, we generated soluble forms of different Vβ domains with a high affinity for binding superantigens. However, a broader spectrum antagonist is required for the neutralization of multiple toxins. In the present study, we expressed Vβ domains in tandem as a single-chain protein and neutralized the clinically important superantigens staphylococcal enterotoxin B and TSS toxin-1 with a single agent.

Details

Title: Subtitle: Neutralization of Multiple Staphylococcal Superantigens by a Single-Chain Protein Consisting of Affinity-Matured, Variable Domain Repeats
Creators: Xi Yang - Department of Biochemistry, University of Illinois
Rebecca A Buonpane - Department of Biochemistry, University of Illinois
Beenu Moza - Boston Biomedical Research Institute
A. K. M. Nur-ur Rahman - Department of Microbiology and Immunology, University of Western Ontario
Ningyan Wang - Department of Biochemistry, University of Illinois
Patrick M Schlievert - Department of Microbiology, University of Minnesota Medical School
John K McCormick - Department of Microbiology and Immunology, University of Western Ontario
Eric J Sundberg - Boston Biomedical Research Institute
David M Kranz - Department of Biochemistry, University of Illinois
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.198(3), pp.344-348
DOI: 10.1086/589776
PMID: 18522504
PMCID: PMC2649774
NLM abbreviation: J Infect Dis
ISSN: 0022-1899
eISSN: 1537-6613
Publisher: The University of Chicago Press
Language: English
Date published: 08/01/2008
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001152002771

Metrics

21 Record Views

15 Times Cited - Web of Science

See more details