Journal article
Neutralizing antibody to hepatitis A virus in immune serum globulin and in the sera of human recipients of immune serum globulin
Gastroenterology (New York, N.Y. 1943), Vol.89(3), pp.637-642
09/1985
DOI: 10.1016/0016-5085(85)90462-7
PMID: 2991071
Abstract
We determined the titer of neutralizing antibody to hepatitis A virus in five lots of immune serum globulin and in the sera of human recipients of immune globulin using a new and sensitive procedure, the radioimmunofocus inhibition assay. The neutralizing antibody titer of four immune globulin lots ranged from 1:170,000 to 1:406,000, and was ~100-fold the antibody titer determined by radioimmunoassay. The neutralizing antibody titer of a Bureau of Biologies reference immunoglobulin was 1:794,000. Sera collected from 18 healthy men who had undergone prophylaxis with immune globulin (0.02 ml/kg body wt) were negative when tested by radioimmunoassay. However, 2 of 18 sera collected before administration of immune globulin and sera from all 18 men collected 3 and 55 days later contained detectable neutralizing antibody. The measurement of neutralizing antibody should prove useful for standardizing passive immunoprophylaxis against hepatitis A as well as for evaluating the potential efficacy of new hepatitis A virus vaccines.
Details
- Title: Subtitle
- Neutralizing antibody to hepatitis A virus in immune serum globulin and in the sera of human recipients of immune serum globulin
- Creators
- Jack T StapletonRobert JansenStanley M Lemon
- Resource Type
- Journal article
- Publication Details
- Gastroenterology (New York, N.Y. 1943), Vol.89(3), pp.637-642
- DOI
- 10.1016/0016-5085(85)90462-7
- PMID
- 2991071
- NLM abbreviation
- Gastroenterology
- ISSN
- 0016-5085
- eISSN
- 1528-0012
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 09/1985
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094876802771
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