Journal article
Neutron-encoded mass signatures for multiplexed proteome quantification
Nature methods, Vol.10(4), pp.332-334
04/2013
DOI: 10.1038/nmeth.2378
PMCID: PMC3612390
PMID: 23435260
Abstract
We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.
Details
- Title: Subtitle
- Neutron-encoded mass signatures for multiplexed proteome quantification
- Creators
- Alexander S Hebert - Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USAAnna E MerrillDerek J BaileyAmelia J StillMichael S WestphallEric R StrieterDavid J PagliariniJoshua J Coon
- Resource Type
- Journal article
- Publication Details
- Nature methods, Vol.10(4), pp.332-334
- DOI
- 10.1038/nmeth.2378
- PMID
- 23435260
- PMCID
- PMC3612390
- NLM abbreviation
- Nat Methods
- ISSN
- 1548-7091
- eISSN
- 1548-7105
- Publisher
- United States
- Grant note
- R01 GM080148 / NIGMS NIH HHS 5T32GM007215-37 / NIGMS NIH HHS 5T32HG002760 / NHGRI NIH HHS T32 GM007215 / NIGMS NIH HHS T32 HG002760 / NHGRI NIH HHS
- Language
- English
- Date published
- 04/2013
- Academic Unit
- Pathology; Injury Prevention Research Center
- Record Identifier
- 9984047612202771
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