Neutron-encoded protein quantification by peptide carbamylation

Arne Ulbrich; Anna E Merrill; Alexander S Hebert; Michael S Westphall; Mark P Keller; Alan D Attie; Joshua J Coon

doi:10.1007/s13361-013-0765-z

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Neutron-encoded protein quantification by peptide carbamylation

Journal article

Open access

Peer reviewed

Neutron-encoded protein quantification by peptide carbamylation

Arne Ulbrich, Anna E Merrill, Alexander S Hebert, Michael S Westphall, Mark P Keller, Alan D Attie and Joshua J Coon

Journal of the American Society for Mass Spectrometry, Vol.25(1), pp.6-9

01/2014

DOI: 10.1007/s13361-013-0765-z

PMCID: PMC3880647

PMID: 24178922

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http://doi.org/10.1007/s13361-013-0765-zView

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Abstract

We describe a chemical tag for duplex proteome quantification using neutron encoding (NeuCode). The method utilizes the straightforward, efficient, and inexpensive carbamylation reaction. We demonstrate the utility of NeuCode carbamylation by accurately measuring quantitative ratios from tagged yeast lysates mixed in known ratios and by applying this method to quantify differential protein expression in mice fed a either control or high-fat diet.

Isotope Labeling - methods

Animals

Diet, High-Fat

Mass Spectrometry

Neutrons

Mice

Proteins - analysis

Proteins - chemistry

Proteome - analysis

Proteomics - methods

Proteome - metabolism

Details

Title: Subtitle: Neutron-encoded protein quantification by peptide carbamylation
Creators: Arne Ulbrich - Department of Chemistry, University of Wisconsin, Madison, WI, 53706, USA
Anna E Merrill
Alexander S Hebert
Michael S Westphall
Mark P Keller
Alan D Attie
Joshua J Coon
Resource Type: Journal article
Publication Details: Journal of the American Society for Mass Spectrometry, Vol.25(1), pp.6-9
DOI: 10.1007/s13361-013-0765-z
PMID: 24178922
PMCID: PMC3880647
NLM abbreviation: J Am Soc Mass Spectrom
ISSN: 1044-0305
eISSN: 1879-1123
Publisher: United States
Grant note: T32 HG002760 / NHGRI NIH HHS R01 DK066369 / NIDDK NIH HHS R01 GM080148 / NIGMS NIH HHS DK091207 / NIDDK NIH HHS R24 DK091207 / NIDDK NIH HHS R01 DK058037 / NIDDK NIH HHS 5T32HG002760 / NHGRI NIH HHS GM080148 / NIGMS NIH HHS DK058037 / NIDDK NIH HHS
Language: English
Date published: 01/2014
Academic Unit: Pathology; Injury Prevention Research Center
Record Identifier: 9984047775002771

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