Neutrophil-derived extracellular vesicles modulate the phenotype of naïve human neutrophils

Maya F Amjadi; Benjamin S Avner; Mallary C Greenlee-Wacker; Alexander R Horswill; William M Nauseef

doi:10.1002/JLB.3AB0520-339RR

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Neutrophil-derived extracellular vesicles modulate the phenotype of naïve human neutrophils

Journal article

Peer reviewed

Neutrophil-derived extracellular vesicles modulate the phenotype of naïve human neutrophils

Maya F Amjadi, Benjamin S Avner, Mallary C Greenlee-Wacker, Alexander R Horswill and William M Nauseef

Journal of leukocyte biology, Vol.110(5), pp.917-925

11/2021

DOI: 10.1002/JLB.3AB0520-339RR

PMCID: PMC8423865

PMID: 33682200

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Abstract

Neutrophils (PMN) regulate inflammation in many ways, including communication with other immune cells via extracellular vesicles (EVs). EVs released by human neutrophils activated with N-formylmethionyl-leucyl-phenylalanine (fMLF) (PMN-fMLF EVs) had an outside-out orientation and contained functionally important neutrophil plasma membrane proteins, including flavocytochrome b558, and enzymatically active granule proteins, elastase, and myeloperoxidase. Treatment of naïve PMN with PMN-fMLF EVs primed fMLF-stimulated NADPH oxidase activity, increased surface expression of the complement receptors CD11b/CD18 and CD35, the specific granule membrane protein CD66, and flavocytochrome b , and promoted phagocytosis of serum-opsonized Staphylococcus aureus. The primed oxidase activity reflected increased surface expression of flavocytochrome b558 and phosphorylation of SER345 in p47 , two recognized mechanisms for oxidase priming. Taken together, these data demonstrate that stimulated PMN released EVs that altered the phenotype of naïve phagocytes by priming of the NADPH oxidase activity and augmenting phagocytosis, two responses that are integral to optimal PMN host defense.

Extracellular Vesicles - immunology

Extracellular Vesicles - metabolism

Humans

NADPH Oxidases - metabolism

Neutrophils - immunology

Neutrophils - metabolism

Phagocytosis - immunology

Phenotype

Details

Title: Subtitle: Neutrophil-derived extracellular vesicles modulate the phenotype of naïve human neutrophils
Creators: Maya F Amjadi - University of Iowa
Benjamin S Avner - University of Iowa
Mallary C Greenlee-Wacker - Central Michigan University
Alexander R Horswill - University of Colorado Denver
William M Nauseef - University of Iowa
Resource Type: Journal article
Publication Details: Journal of leukocyte biology, Vol.110(5), pp.917-925
DOI: 10.1002/JLB.3AB0520-339RR
PMID: 33682200
PMCID: PMC8423865
ISSN: 0741-5400
eISSN: 1938-3673
Grant note: R01 AI132335 / NIAID NIH HHS P30 ES005605 / NIEHS NIH HHS P01 AI083211 / NIAID NIH HHS I01 BX000513 / BLRD VA R15 GM132992 / NIGMS NIH HHS I01 BX002711 / BLRD VA R01 AI116546 / NIAID NIH HHS T32 AG000213 / NIA NIH HHS
Language: English
Date published: 11/2021
Academic Unit: Infectious Diseases; Internal Medicine
Record Identifier: 9985237503302771

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