Neutrophil involvement in cross-priming CD8+ T cell responses to bacterial antigens

Amy R Tvinnereim; Sara E Hamilton; John T Harty

doi:10.4049/jimmunol.173.3.1994

Back

Neutrophil involvement in cross-priming CD8+ T cell responses to bacterial antigens

Journal article

Open access

Peer reviewed

Neutrophil involvement in cross-priming CD8+ T cell responses to bacterial antigens

Amy R Tvinnereim, Sara E Hamilton and John T Harty

The Journal of immunology (1950), Vol.173(3), pp.1994-2002

08/01/2004

DOI: 10.4049/jimmunol.173.3.1994

PMID: 15265934

View Online

Abstract

Substantial CD8(+) T cell responses are generated after infection of mice with recombinant Listeria monocytogenes strains expressing a model epitope (lymphocytic choriomeningitis virus NP(118-126)) in secreted and nonsecreted forms. L. monocytogenes gains access to the cytosol of infected cells, where secreted Ags can be accessed by the endogenous MHC class I presentation pathway. However, the route of presentation of the nonsecreted Ag in vivo remains undefined. In this study we show that neutrophil-enriched peritoneal exudate cells from L. monocytogenes-infected mice can serve as substrates for in vitro cross-presentation of both nonsecreted and secreted Ag by dendritic cells as well as for in vivo cross-priming of CD8(+) T cells. In addition, specific neutrophil depletion in vivo by low dose treatment with either of two Ly6G-specific mAb substantially decreased the relative CD8(+) T cell response against the nonsecreted, but not the secreted, Ag compared with control Ab-treated mice. Thus, neutrophils not only provide rapid innate defense against infection, but also contribute to shaping the specificity and breadth of the CD8(+) T cell response. In addition, cross-presentation of bacterial Ags from neutrophils may explain how CD8(+) T cell responses are generated against Ags from extracellular bacterial pathogens.

Recombinant Fusion Proteins - immunology

Dendritic Cells - immunology

Nucleoproteins - genetics

Antigens, Bacterial - immunology

Adoptive Transfer

Listeria monocytogenes - immunology

Antigen Presentation

Peptide Fragments - immunology

Female

Ascitic Fluid - cytology

Cells, Cultured - immunology

Peptide Fragments - genetics

Antibodies, Monoclonal - immunology

Nucleoproteins - immunology

Mice, Inbred C57BL

Neutrophils - immunology

H-2 Antigens - immunology

Animals

Antigens, Ly - immunology

Neutrophils - transplantation

Listeria monocytogenes - genetics

T-Cell Antigen Receptor Specificity

Cytosol - microbiology

Mice

Mice, Inbred BALB C

CD8-Positive T-Lymphocytes - immunology

Immunity, Cellular

Crosses, Genetic

Details

Title: Subtitle: Neutrophil involvement in cross-priming CD8+ T cell responses to bacterial antigens
Creators: Amy R Tvinnereim - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Sara E Hamilton
John T Harty
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.173(3), pp.1994-2002
Publisher: United States
DOI: 10.4049/jimmunol.173.3.1994
PMID: 15265934
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI42767 / NIAID NIH HHS AI50073 / NIAID NIH HHS AI46653 / NIAID NIH HHS AI07260 / NIAID NIH HHS AI07511 / NIAID NIH HHS
Language: English
Date published: 08/01/2004
Academic Unit: Pathology
Record Identifier: 9984047877902771

Metrics

26 Record Views

112 Times Cited - Web of Science

See more details